The success of a dental implant relies on the presence of an optimal alveolar ridge. The aim of this study was to fabricate HPMC crosslinked chitosan based scaffolds for alveolar bone repair. Our results indicated that HPMC crosslinked CH/BG foams presented better morphological structure (132-90.5 μm) and mechanical responses (0.451 MPa with 100 mg BG) as confirmed by SEM analysis and fatigue testing respectively. Cytotoxicity analysis at day 2, 4 and 8 demonstrated that all composites were non-toxic and supported cellular viability. Calcein AM/propidium iodide staining, Hoechst nuclear staining and cell adhesion assay reiterated that scaffolds supported pre-osteoblast cell growth, adhesion and proliferation. Differentiation potential of pre-osteoblast cells was enhanced as confirmed by alkaline phosphate assay. Furthermore, loss of S. aureus viability as low as 35% was attributed to synergistic effects of components. Overall, our results suggest that HPMC crosslinked scaffolds are potential candidates for alveolar bone repair.

牙植入物的成功取决于最佳牙槽的存在。这项研究的目的是制造用于牙槽骨修复的HPMC交联壳聚糖基支架。我们的结果表明，HPMC交联的CH / BG泡沫表现出更好的形态结构（132-90.5μm）和机械响应（0.451 MPa，100 mg BG），分别由SEM分析和疲劳测试证实。第2、4和8天的细胞毒性分析表明，所有复合材料均无毒并支持细胞生存能力。钙黄绿素AM /碘化丙啶染色，Hoechst核染色和细胞粘附测定法重申，支架支持成骨前细胞的生长，粘附和增殖。如碱性磷酸盐测定所证实的，前成骨细胞的分化潜能得以增强。此外，损失小号。金黄色葡萄球菌的存活率低至35％归因于各成分的协同作用。总体而言，我们的结果表明HPMC交联支架是肺泡骨修复的潜在候选者。