Recent study has shown that RNA interference (RNAi) is efficient in emerald ash borer (EAB), Agrilus planipennis, and that ingestion of double-stranded RNA (dsRNA) targeting specific genes causes gene silencing and mortality in neonates. Here, we report on the identification of highly effective target genes for RNAi-mediated control of EAB. We screened 13 candidate genes in neonate larvae and selected the most effective target genes for further investigation, including their effect on EAB adults and on a non-target organism, Tribolium castaneum. The two most efficient target genes selected, hsp (heat shock 70-kDa protein cognate 3) and shi (shibire), caused up to 90% mortality of larvae and adults. In EAB eggs, larvae, and adults, the hsp is expressed at higher levels when compared to that of shi. Ingestion of dsHSP and dsSHI caused mortality in both neonate larvae and adults. Administration of a mixture of both dsRNAs worked better than either dsRNA by itself. In contrast, injection of EAB.dsHSP and EAB.dsSHI did not cause mortality in T. castaneum. Thus, the two genes identified cause high mortality in the EAB with no apparent phenotype effects in a non-target organism, the red flour beetle, and could be used in RNAi-mediated control of this invasive pest.

中文翻译：

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鉴定高效靶基因，用​​于RNAi介导的翡翠灰boreAgrilus planipennis的控制。

最近的研究表明，RNA干扰（RNAi）在翡翠bore（EAB），姬松茸（Agrilus planipennis）中是有效的，并且针对特定基因的双链RNA（dsRNA）的摄入会导致新生儿的基因沉默和死亡。在这里，我们报告的RNAi介导的EAB控制高效目标基因的鉴定。我们筛选了新生幼虫中的13个候选基因，并选择了最有效的靶基因进行进一步研究，包括它们对EAB成虫和非靶生物organism藜的影响。选择的两个最有效的靶基因hsp（热休克70-kDa蛋白同源3）和shi（抑制物）导致幼虫和成虫的死亡率高达90％。在EAB卵，幼虫和成虫中，与shi相比，hsp的表达水平更高。摄入dsHSP和dsSHI会导致新生儿幼虫和成人死亡。两种dsRNA混合物的管理效果都优于任何一种dsRNA本身。相反，注射EAB.dsHSP和EAB.dsSHI不会导致锥栗木的死亡。因此，鉴定出的两个基因在EAB中导致高死亡率，而在非靶标生物（红色面粉甲虫）中没有明显的表型效应，可用于RNAi介导的这种入侵性有害生物的控制。