Thrombocytopenia or chronic depletion of platelets in blood, could create life-threatening conditions in patients who receive aggressive systemic radiation and chemotherapy. Currently there are no approved agents for the rapid treatment of thrombocytopenia. In the present study, we demonstrate that administration of Orientin, a glycosidic flavonoid or dietary administration of Orientin containing Tulsi (Holy Basil) leaves, results in a significant increase in circulating platelets in a clinically relevant mouse model. No noticeable effects were observed on red blood cells, white blood cells or other hematologic parameters in treated animals indicating that Orientin specificity enhances platelet formation. The gene expression and immunophenotyping of bone marrow revealed that Orientin stimulates megakaryopoiesis specific transcriptional program. A significant increase in colony formation in bone marrow cells from Orientin pretreated mice further complemented the effect of Orientin on progenitor cells. The ex-vivo differentiation of irradiated human peripheral blood CD34⁺ stem cells demonstrated stimulatory effects of Orientin on megakaryocyte erythrocyte progenitors (MEP). The results show that Orientin, a non-toxic readily available natural product can counter platelet imbalances. Thrombocytopenia also develop as a consequence of multiple hematologic malignancies and side effects of treatments. Dietary supplementation of Orientin containing phytochemicals could be effective as countermeasures and viable therapeutics.

中文翻译：

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Ocimum黄酮Orientin作为血小板减少症的对策。

血小板减少症或血液中血小板的慢性耗竭，可能会在接受积极的全身放疗和化疗的患者中造成危及生命的状况。目前尚无批准的用于血小板减少症快速治疗的药物。在本研究中，我们证明了Orientin（一种糖苷类黄酮）的施用或Orientin的饮食中含有Tulsi（圣罗勒）叶的施用在临床相关的小鼠模型中导致循环血小板的显着增加。在治疗动物中未观察到对红细胞，白细胞或其他血液学参数的明显影响，表明Orientin特异性可增强血小板形成。骨髓的基因表达和免疫表型研究表明，Orientin刺激了巨核细胞的特异性转录程序。Orientin预处理小鼠骨髓细胞中集落形成的显着增加进一步补充了Orientin对祖细胞的作用。辐射的人外周血CD34的离体分化⁺干细胞证明了Orientin对巨核红细胞祖细胞（MEP）的刺激作用。结果表明，Orientin是一种无毒，易于获得的天然产品，可以抵消血小板的不平衡。血小板减少症也是多种血液系统恶性肿瘤和治疗副作用的结果。膳食中补充含有Orientin的植物化学物质可能是有效的对策和可行的治疗方法。