This study aimed to verify the chelating, antioxidant and cytoprotective activities of Psidium brownianum Mart. Ex DC against mercury and aluminum. The ethanolic extract, as well as the tannic and flavonoid fractions, were prepared and subjected to liquid chromatography-mass spectrometry analysis. Ferric ion reduction and antioxidant activity measurement using the FRAP method were performed with P. brownianum. After determining the sub-allelopathic doses, germination tests using Lactuca sativa (lettuce) seeds were performed. The main compounds identified in the extract and fractions were: quercetin and its derivatives; myricetin and its derivatives; gallic acid; ellagic acid; quinic acid and gallocatechin. The Minimum Inhibitory Concentration (MIC) for all samples were ≥ 1024 μg/mL. The flavonoid fraction in association with mercury chloride demonstrated cytoprotection (p < 0.001). The sub-allelopathic concentration used was 64 μg/mL. The extract and fractions were cytoprotective for radicles and caulicles when assayed in association with mercury and against aluminum for radicles. This suggests that the P. brownianum extract and its fractions present cytoprotective activity, possibly related to the antioxidant effect of secondary metabolites, especially flavonoids.

中文翻译：

---

Psidium Brownianum Mart 生物活性产物的 LC-MS 分析及其对汞和铝毒性的细胞保护作用。前 DC。


本研究旨在验证 Psidium Brownianum Mart 的螯合、抗氧化和细胞保护活性。 Ex DC 对汞和铝。制备乙醇提取物以及单宁和类黄酮级分，并进行液相色谱-质谱分析。使用 FRAP 方法对 P. Brownianum 进行铁离子还原和抗氧化活性测量。确定亚化感剂量后，使用生菜种子进行发芽测试。提取物和馏分中鉴定出的主要化合物是：槲皮素及其衍生物；杨梅素及其衍生物；没食子酸；鞣花酸；奎尼酸和没食子儿茶素。所有样品的最低抑菌浓度 (MIC) 均≥ 1024 μg/mL。黄酮类成分与氯化汞结合显示出细胞保护作用（p < 0.001）。使用的亚化感浓度为 64 μg/mL。当与汞和铝一起对胚根进行测定时，提取物和级分对胚根和花茎具有细胞保护作用。这表明 P. Brownianum 提取物及其组分具有细胞保护活性，可能与次级代谢产物（尤其是类黄酮）的抗氧化作用有关。