There is an increased interest in incorporating multi-wall carbon nanotubes (MWCNTs) into polymer matrices to control the adhesion of bacteria to surfaces and the subsequent formation of biofilm growth on the surface of water pipes, food packages, and medical devices. Microbial interactions with carbon nanotube-polymer composites in the environment are not well understood. The growth of Pseudomonas fluorescens (gram-negative) and Mycobacterium smegmatis (gram-positive) biofilms on copper, polyethylene (PE), polyvinyl chloride, and stainless steel was compared with growth on MWCNT-PE composites in order to gain insight into the effect of the surface properties of nanomaterials on the attachment and proliferation of microorganism which could result in the engineering of better, non-fouling materials. A statistical analysis of the biofilm growth showed a significant impact of materials for both P. fluorescens (p < 0.0001) and M. smegmatis (p = 0.00426). Biofilm growth after 56 days on PE compared to biofilm growth on copper surfaces decreased by 46.4% and 34.9% for P. fluorescens and M. smegmatis, respectively. Biofilm growth on PE-multiwall-carbon-nanotubes (MWCNTs)-composites surface compared to PE decreased by 89.3% and 29% for P. fluorescens and M. smegmatis, respectively. Bacterial species (p < 0.0006) and surface roughness (p < 0.0001) were important factors in determining the attachment and initial biofilm growth rate. The interactions between cells and material surface could be attributed to the complicated and collective effect of electrostatic forces, hydrophobic interactions, and hydrogen/covalent bonding. Further study is needed to determine whether or not there is a difference between the cell attachment in the exponential growth phase and the stationary, or decay, phase cells.

人们越来越关注将多壁碳纳米管（MWCNT）掺入聚合物基质中，以控制细菌与表面的粘附以及随后在水管，食品包装和医疗设备表面上形成生物膜的过程。与环境中的碳纳米管-聚合物复合物的微生物相互作用尚不十分清楚。荧光假单胞菌（革兰氏阴性）和耻垢分枝杆菌的生长将铜，聚乙烯（PE），聚氯乙烯和不锈钢上的（革兰氏阳性）生物膜与MWCNT-PE复合材料上的生长进行了比较，以了解纳米材料的表面性质对微生物附着和增殖的影响可以设计出更好的防污材料。对生物膜生长的统计分析表明，两种材料对P的影响都很大。荧光假单胞菌（p  <0.0001）和中号。耻垢分枝杆菌（p  = 0.00426）。与铜表面的生物膜生长相比，PE上56天后的生物膜生长下降了46.4％，而P的生物膜生长下降了34.9％。荧光素和中号。包皮垢。与PE相比，PE-多壁碳纳米管（MWCNTs）复合材料表面的生物膜生长减少了89.3％，而P减少了29％。荧光和中号。包皮垢。细菌种类（p  <0.0006）和表面粗糙度（p <0.0001）是确定附着力和初始生物膜生长速率的重要因素。细胞与材料表面之间的相互作用可归因于静电力，疏水相互作用和氢/共价键的复杂而集体的作用。需要进一步研究以确定指数生长期的细胞附着与静止或衰变阶段的细胞之间是否存在差异。