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A disposable on-chip microvalve and pump for programmable microfluidics†
Lab on a Chip ( IF 6.1 ) Pub Date : 2018-03-22 00:00:00 , DOI: 10.1039/c8lc00003d Sung B. Im 1, 2, 3, 4, 5 , M. Jalal Uddin 1, 2, 3, 4, 5 , Gyeong J. Jin 1, 2, 3, 4, 5 , Joon S. Shim 1, 2, 3, 4, 5
Lab on a Chip ( IF 6.1 ) Pub Date : 2018-03-22 00:00:00 , DOI: 10.1039/c8lc00003d Sung B. Im 1, 2, 3, 4, 5 , M. Jalal Uddin 1, 2, 3, 4, 5 , Gyeong J. Jin 1, 2, 3, 4, 5 , Joon S. Shim 1, 2, 3, 4, 5
Affiliation
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In this work, a low-cost PDMS micro-pump and -valve have been designed and developed to control multiple reagents for enzyme-linked immunosorbent assay (ELISA) on a programmable lab-on-a-chip (LOC) platform. The micro pump and valves were precisely controlled by selectively pressurizing the PDMS channels and chamber to actuate the multiple reagents in a controlled manner. Selective pressurizing of the PDMS structures was initiated by a simple system that maneuvered a single roller bar operated by a programmed microprocessor. The performance of the micro-pump was fully characterized and a minimum fluid volume of 1 μL was controlled. Also, the on-chip microvalves were programmed to flow the multiple reagents to automatically process the multi-step ELISA procedures. By applying the proposed platform, 19.40 pg ml−1 cardiac troponin T (cTnT) was successfully detected on the LOC device by using multiple programmed valves as multiple steps of the enzyme-linked sandwich immunoassay. As a result, the developed micro-pump and -valve, which were successfully applied to actuate a series of solutions in a controlled manner, can be widely applied to lab-on-a-chip based bioassays.
中文翻译：
用于可编程微流体的一次性片上微型阀和泵†
在这项工作中，已经设计并开发了一种低成本的PDMS微泵和阀门，以在可编程芯片实验室（LOC）平台上控制用于酶联免疫吸附测定（ELISA）的多种试剂。通过选择性地加压PDMS通道和腔室，以可控方式驱动多种试剂，可以精确控制微型泵和阀门。PDMS结构的选择性加压是通过一个简单的系统启动的，该系统可操纵由编程的微处理器操作的单个压杆。充分表征了微型泵的性能，并控制了1μL的最小流体体积。同样，对片上微型阀进行编程以使多种试剂流动以自动处理多步骤ELISA程序。通过应用建议的平台，可达到19.40 pg ml -1通过使用多个程序化阀门作为酶联三明治免疫测定的多个步骤，在LOC设备上成功检测到了心肌肌钙蛋白T（cTnT）。结果，成功开发的微型泵和阀门以可控方式启动了一系列解决方案，可广泛应用于基于芯片实验室的生物测定。
更新日期：2018-03-22
中文翻译：
用于可编程微流体的一次性片上微型阀和泵†
在这项工作中，已经设计并开发了一种低成本的PDMS微泵和阀门，以在可编程芯片实验室（LOC）平台上控制用于酶联免疫吸附测定（ELISA）的多种试剂。通过选择性地加压PDMS通道和腔室，以可控方式驱动多种试剂，可以精确控制微型泵和阀门。PDMS结构的选择性加压是通过一个简单的系统启动的，该系统可操纵由编程的微处理器操作的单个压杆。充分表征了微型泵的性能，并控制了1μL的最小流体体积。同样，对片上微型阀进行编程以使多种试剂流动以自动处理多步骤ELISA程序。通过应用建议的平台，可达到19.40 pg ml -1通过使用多个程序化阀门作为酶联三明治免疫测定的多个步骤，在LOC设备上成功检测到了心肌肌钙蛋白T（cTnT）。结果，成功开发的微型泵和阀门以可控方式启动了一系列解决方案，可广泛应用于基于芯片实验室的生物测定。
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