We identified a new cyclic lipodepsipeptide, cystargamide B (1), from the mycelial extract of a Kaempferia galanga rhizome-derived actinomycete strain, Streptomyces sp. PB013. The planar structure was elucidated based on high resolution fast-atom bombardment mass spectrometry (HRFABMS) spectroscopy and one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR) spectroscopic data. The absolute configurations of the constituent amino acids were determined using advanced Marfey's method. Cystargamide B (1) includes rare structural units: a 5-hydroxytryptophan residue and a 2,3-epoxy fatty acid side chain. Notably, cystargamide B (1) inhibited the protease activity of the NS2B/NS3 complex from dengue virus.

中文翻译：

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Cystargamide B，一种环脂二肽，具有Streptomyces sp。的蛋白酶抑制活性。

我们从Kaempferia galanga根茎来源的放线菌菌株Streptomyces sp。的菌丝体提取物中鉴定了一种新的环状脂肽肽，cystargamide B（1）。PB013。基于高分辨率快速原子轰击质谱（HRFABMS）光谱学和一维（1D）和二维（2D）核磁共振（NMR）光谱数据阐明了平面结构。组成氨基酸的绝对构型使用先进的Marfey方法确定。Cystargamide B（1）包含稀有结构单元：5-羟基色氨酸残基和2,3-环氧脂肪酸侧链。值得注意的是，胱氨酰胺B（1）抑制了来自登革热病毒的NS2B / NS3复合物的蛋白酶活性。