Prebiotic effects of resistant starch from purple yam (Dioscorea alata L.) on the tolerance and proliferation ability of Bifidobacterium adolescentis in vitro,Food & Function

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Prebiotic effects of resistant starch from purple yam (Dioscorea alata L.) on the tolerance and proliferation ability of Bifidobacterium adolescentis in vitro
Food & Function ( IF 5.1 ) Pub Date : 2018-03-22 00:00:00 , DOI: 10.1039/c7fo01919j
Tao Li _{1,

2,

3,

4} , Lei Chen _{1,

2,

3,

4} , Jianbo Xiao _{1,

2,

3,

4} , Fengping An _{1,

2,

3,

4} , Cheng Wan _{1,

2,

3,

4} , Hongbo Song _{1,

2,

3,

4,

5}

Affiliation

The in vitro prebiotic effects of resistant starch (RS), prepared by different treatments from purple yam, on Bifidobacterium adolescentis (bifidobacteria for short), were investigated. Tolerance tests indicated that bifidobacteria in PDS (prepared by debranching combined with autoclaving) and PDS.H (PDS further treated by double enzyme hydrolysis) media adapted better to simulated upper gastrointestinal conditions (at pH 1.5–3.0 and 0.3% and 1.0% bile acid) than those in GLU (glucose) and DAS (prepared by autoclaving) media. PDS.H, which had the highest digestion resistibility, exhibited significant effects on the OD_{600 nm} value (1.544) and the pH value (4.21) when the carbohydrate concentration was 20 g L⁻¹. Additionally, the exponential growth phase of bifidobacteria was 2 h in the PDS or PDS.H media, whereas it was 4 h in the GLU or DAS media. A higher content of short-chain fatty acids (SCFAs) was obtained in the PDS.H medium. Analysis of the structural features of RS and fermented RS indicated that PDS, especially PDS.H, had a rougher surface and higher crystallinity than DAS. Fermented RS in a simulated large bowel environment showed an eroded surface and decreased crystallinity. All of these findings suggest that RS with a rough surface and perfect crystalline structure could protect bifidobacteria from gastrointestinal conditions and enhance the proliferation of bifidobacteria.

中文翻译：

从紫山药（抗性淀粉的益生元效果参薯L.）上的耐受性和增殖能力青春双歧杆菌在体外

在体外的抗性淀粉（RS）益生元效果，通过不同的处理从紫山药制备，上青春双歧杆菌（简称双歧杆菌），进行了研究。耐受性测试表明，PDS（通过脱支结合高压灭菌制备）和PDS.H（通过双酶水解进一步处理的PDS）培养基中的双歧杆菌更好地适应了模拟的上消化道条件（在pH 1.5-3.0和0.3％和1.0％的胆汁酸下）），而不是GLU（葡萄糖）和DAS（通过高压灭菌制备）培养基中的。当碳水化合物浓度为20 g L ^-1时，具有最高耐消化性的PDS.H对OD _{600 nm}值（1.544）和pH值（4.21）表现出显着影响。此外，双歧杆菌的指数生长期在PDS或PDS.H培养基中为2 h，而在GLU或DAS培养基中为4 h。在PDS.H培养基中获得了更高含量的短链脂肪酸（SCFA）。对RS和发酵RS的结构特征的分析表明，PDS，特别是PDS.H，具有比DAS更粗糙的表面和更高的结晶度。在模拟的大肠环境中发酵的RS显示出腐蚀的表面并降低了结晶度。所有这些发现表明，具有粗糙表面和完美晶体结构的RS可以保护双歧杆菌免受胃肠道疾病的侵害，并增强双歧杆菌的增殖。

更新日期：2018-03-22

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