Silk Fibroin Acts as a Self-Emulsifier to Prepare Hierarchically Porous Silk Fibroin Scaffolds through Emulsion–Ice Dual Templates,ACS Omega

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Silk Fibroin Acts as a Self-Emulsifier to Prepare Hierarchically Porous Silk Fibroin Scaffolds through Emulsion–Ice Dual Templates
ACS Omega ( IF 3.7 ) Pub Date : 2018-03-21 00:00:00 , DOI: 10.1021/acsomega.7b01874
Jianchuan Wen ₁ , Jinrong Yao ₁ , Xin Chen ₁ , Zhengzhong Shao ₁

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Silk fibroin (SF) has shown enormous potentials in various fields; however, application of SF in emulsion technology is quite limited. Here, we use SF as a self-emulsifier to form an oil-in-water (O/W) emulsion by emulsifying 1-butanol in SF aqueous solution. This showed that SF possessed strong surface activity to stabilize the O/W emulsion without the need for any other surface-active agent until its solidification because of 1-butanol-induced conformational transition of SF to β-sheet. After freezing the preformed emulsions at −20 °C, robust three-dimensional porous SF scaffolds were prepared without the need for any further post-treatment. The evolution from the O/W emulsion to porous scaffold formation under freezing was tracked, and an emulsion–ice dual template mechanism was proposed for scaffold formation, based on which SF scaffolds with controllable hierarchically porous structures were achieved by tuning the dispersed droplet volume fraction. Furthermore, SF scaffolds with hierarchical porosity showed significantly higher bioactivity toward L929 fibroblasts than that of SF scaffolds with mono macroporosity, highlighting the great asset of this hierarchically porous SF scaffold for broad applications in tissue engineering. Therefore, the strong surface-active characteristic of SF presented here, in addition to its distinct advantages, sheds a bright light on the application of SF in the vast range of emulsion technologies, especially in cosmetic-, food-, and biomedical-related areas.

中文翻译：

丝素蛋白充当自乳化剂，通过乳液-冰双重模板制备分层多孔的丝素蛋白支架

丝素蛋白（SF）在各个领域都显示出巨大的潜力。然而，SF在乳液技术中的应用是非常有限的。在这里，我们使用SF作为自乳化剂，通过在SF水溶液中乳化1-丁醇来形成水包油（O / W）乳液。这表明由于1-丁醇诱导的SF向β-片层的构象转变，SF具有强大的表面活性来稳定O / W乳液，直到其固化之前不需要任何其他表面活性剂。将预制的乳液在-20°C下冷冻后，无需任何进一步的后处理即可制备坚固的三维多孔SF支架。跟踪了从O / W乳液到冷冻状态下多孔支架形成的演变，并提出了乳液-冰双重模板机制来形成支架，基于此，通过调节分散的液滴体积分数可实现具有可控的分层多孔结构的SF支架。此外，具有多孔性的SF支架比具有单大孔性的SF支架对L929成纤维细胞显示出显着更高的生物活性，这突显了这种具有分层多孔性的SF支架在组织工程中的广泛应用的巨大资产。因此，这里展示的SF强大的表面活性特性，除了其独特的优势外，还为SF在各种乳液技术中的应用提供了亮点，特别是在化妆品，食品和生物医学相关领域。具有分级孔隙度的SF支架对L929成纤维细胞的生物活性明显高于具有单一大孔性的SF支架，这突显了这种分层多孔的SF支架在组织工程中的广泛应用的巨大资产。因此，这里展示的SF强大的表面活性特性，除了其独特的优势外，还为SF在各种乳液技术中的应用提供了亮点，特别是在化妆品，食品和生物医学相关领域。具有分级孔隙度的SF支架对L929成纤维细胞的生物活性明显高于具有单一大孔性的SF支架，这突显了这种分层多孔的SF支架在组织工程中的广泛应用的巨大资产。因此，这里展示的SF强大的表面活性特性，除了其独特的优势外，还为SF在各种乳液技术中的应用提供了亮点，特别是在化妆品，食品和生物医学相关领域。

更新日期：2018-03-21

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