Modern microflow cytometers are sophisticated instruments capable of measuring multiple physical characteristics of a single cell simultaneously as the cells flow in suspension through a measuring device. Cytometers are the tool of choice for the high-speed acquisition and analysis of large single cell populations. However, traditional devices lack the ability to provide intracellular spatial information. In the past few decades, various flow cytometer systems with the ability to combine cell/particle detection and the acquisition of two or three-dimensional spatial information have been proposed. However, these devices suffer a number of drawbacks Accordingly, the problem of developing more sophisticated microflow cytometers based on electrical impedance, optical detection and image analysis methods has received significant attention in the literature. This review describes some of the major advances made in the microfluidic cytometry field over the past ten years. The review focuses specifically on recent proposals for enhanced microfluidic focusing techniques and detection/analysis methods, respectively. Overall, the review provides a useful insight into the microflow cytometer technology field for both new and existing users.

现代微流式细胞仪是复杂的仪器，能够在细胞悬浮在悬浮液中流过测量装置的同时测量单个细胞的多种物理特性。细胞计数仪是用于大型单细胞群体的高速采集和分析的首选工具。但是，传统设备缺乏提供细胞内空间信息的能力。在过去的几十年中，已经提出了具有将细胞/颗粒检测与二维或三维空间信息的采集相结合的能力的各种流式细胞仪系统。然而，这些装置具有许多缺点。因此，存在基于电阻抗开发更复杂的微流式细胞仪的问题，光学检测和图像分析方法在文献中受到了极大的关注。这篇综述描述了过去十年在微流式细胞术领域取得的一些重大进展。审查专门针对增强微流聚焦技术和检测/分析方法的最新建议，分别。总体而言，该评论为新用户和现有用户提供了对微流式细胞仪技术领域的有用见解。