Functionalized phenanthridine based probe has been rationally designed and synthesized. ¹H & ¹³NMR, X-ray photoelectron spectra and HRMS were conducted to characterize and prove its structure. The probe exhibited fluorogenic selectivity to detection of Ru³⁺ among the metal ions tested. The probe showed linear fluorescence increase over the introduction of Ru³⁺. The fluorescence increase attributed to the complex formation between probe 2 and Ru³⁺. Job plot experiments showed that there was 1:1 complex formation between receptor 2 and Ru³⁺. Further the recognition of Ru³⁺ ion in the living cell was achieved using fluoresce microscope.

中文翻译：

---

用于荧光启动反应检测Ru ³⁺离子的高选择性化学传感器及其在活细胞中的生物成像识别

功能化的菲啶基探针已得到合理设计和合成。^进行了1 H和¹³ NMR，X射线光电子能谱和HRMS的表征和证明其结构。该探针对检测到的金属离子中的Ru ^3+具有荧光选择性。探针显示出Ru ³⁺引入后线性荧光增加。荧光的增加归因于探针2和Ru ³⁺之间形成复合物。作业图实验表明，受体2和Ru ³⁺之间形成1：1的络合物。进一步认识Ru ³⁺ 活细胞中的离子是使用荧光显微镜获得的。