Abstract In this work, a novel electrochemical biosensor for 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) was developed based on cytochrome P450 2E1 (CYP2E1). The biosensor was fabricated by immobilization of CYP2E1 with poly (diallyldimethylammonium chloride) (PDDA) - poly (sodium-p-styrenesulfonate) (PSS) electrolyte film on a screen-printed carbon electrode (SPCE) electrodeposited with gold nanoparticles (AuNPs). The procedures of electrode modification were characterized by using scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). With the good conductivity of AuNPs as well as the immobilized CYP2E1, the electrochemical performances of the proposed biosensor for the detection of NNK showed a wide linear range of 0–386 μM with a sensitivity of 79 μA cm−2 mM−1 and a low detection limit of 7.71 μM. Additionally, excellent selectivity and reproducibility were also obtained. The fabricated NNK electrochemical biosensor was successfully employed in urine sample determination, exhibiting great potential in the real sample analysis.

中文翻译：

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使用细胞色素 P450 2E1 装饰生物传感器电化学检测 4-(甲基亚硝胺)-1-(3-吡啶基)-1-丁酮

摘要 在这项工作中，基于细胞色素 P450 2E1 (CYP2E1) 开发了一种新型的 4-(甲基亚硝胺)-1-(3-吡啶基)-1-丁酮 (NNK) 电化学生物传感器。该生物传感器是通过将 CYP2E1 与聚（二烯丙基二甲基氯化铵）（PDDA）-聚（对苯乙烯磺酸钠）（PSS）电解质膜固定在丝网印刷碳电极（SPCE）上制备的，该电极与金纳米粒子（AuNPs）电沉积。通过使用扫描电子显微镜（SEM）和电化学阻抗谱（EIS）表征电极改性的过程。由于 AuNPs 的良好导电性以及固定的 CYP2E1，所提出的用于检测 NNK 的生物传感器的电化学性能显示出 0-386 μM 的宽线性范围，灵敏度为 79 μA cm-2 mM-1 和低检测限为 7.71 μM。此外，还获得了出色的选择性和重现性。所制备的NNK电化学生物传感器成功应用于尿样测定，在实际样品分析中显示出巨大的潜力。