Expanding the Chemical Diversity of DNA,Synlett

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Expanding the Chemical Diversity of DNA
Synlett ( IF 1.7 ) Pub Date : 2018-03-20 , DOI: 10.1055/s-0036-1591959
Ryan Hili _{1,

2} , Chun Guo ₁ , Dehui Kong ₁ , Yi Lei ₁

Affiliation

Nucleic acid polymers can be evolved to exhibit desired properties, including molecular recognition of a molecular target and catalysis of a specific reaction. These properties can be readily evolved despite the dearth of chemical diversity available to nucleic acid polymers, especially when compared to the rich chemical complexity of proteins. Expansion of nucleic acid chemical diversity has therefore been an important thrust for improving their properties for analytical and biomedical applications. Herein, we briefly describe the current state-of-the-art for the sequence-defined incorporation of modifications throughout an evolvable nucleic acid polymer. This includes contributions from our own lab, which have expanded the chemical diversity of nucleic acid polymers closer to the level observed in proteinogenic polymers. 1 Introduction 2 Polymerase-Catalyzed Synthesis of Modified Nucleic Acid Polymers 3 Ligase-Catalyzed Oligonucleotide Polymerization (LOOPER) 4 LOOPER with Small Modifications 5 LOOPER with Large Modifications 6 Evolution of Aptamers Derived from LOOPER Libraries 7 Outlook

中文翻译：

扩大 DNA 的化学多样性

核酸聚合物可以进化为表现出所需的特性，包括分子目标的分子识别和特定反应的催化。尽管核酸聚合物缺乏化学多样性，但这些特性很容易进化，尤其是与蛋白质的丰富化学复杂性相比时。因此，核酸化学多样性的扩展是改善其在分析和生物医学应用中的特性的重要推动力。在此，我们简要描述了在整个可进化核酸聚合物中序列定义的修饰掺入的最新技术水平。这包括我们自己实验室的贡献，这些贡献使核酸聚合物的化学多样性更接近于在蛋白质聚合物中观察到的水平。

更新日期：2018-03-20

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