Hospital wastewater is one of the possible sources responsible for antibiotic resistant bacteria spread into the environment. This study proposed a promising strategy, electro-peroxone (E-peroxone) pretreatment followed by a sequencing batch reactor (SBR) for simulated hospital wastewater treatment, aiming to enhance the wastewater treatment performance and to reduce antibiotic resistance genes production simultaneously. The highest chemical oxygen demand (COD) and total organic carbon (TOC) removal efficiency of 94.3% and 92.8% were obtained using the E-peroxone-SBR process. The microbial community analysis through high-throughput sequencing showed that E-peroxone pretreatment could guarantee microbial richness and diversity in SBR, as well as reduce the microbial inhibitions caused by antibiotic and raise the amount of nitrification and denitrification genera. Specially, quantitative real-time PCRs revealed that E-peroxone pretreatment could largely reduce the numbers and contents of antibiotic resistance genes (ARGs) production in the following biological treatment unit. It was indicated that E-peroxone-SBR process may provide an effective way for hospital wastewater treatment and possible ARGs reduction.

医院废水是造成抗生素抗性细菌扩散到环境中的可能来源之一。这项研究提出了一种有前途的策略，即先用电子过氧化物（E-peroxone）预处理，然后再使用测序间歇反应器（SBR）来模拟医院废水，以提高废水处理性能并同时减少抗生素抗性基因的产生。使用E-peroxone-SBR工艺可获得最高的化学需氧量（COD）和总有机碳（TOC）去除效率，分别为94.3％和92.8％。通过高通量测序进行的微生物群落分析表明，E-过氧化物酶预处理可以保证SBR中微生物的丰富性和多样性，以及减少由抗生素引起的微生物抑制，并增加硝化和反硝化属的数量。特别是，定量实时PCR显示，E-过氧化物酶预处理可以大大减少以下生物处理单元中抗生素抗性基因（ARG）的产生数量和含量。结果表明，E-peroxone-SBR工艺可能为医院废水处理和减少ARGs提供有效的方法。