Luminol-reduced gold nanoparticles (LuReGNPs) were prepared and utilized as dual-response signal probes for the development of a colorimetric/chemiluminescent immunochromatographic test strip (ITS). LuReGNPs-labeled antibody was adopted as the signal tracer to conduct immunoassay of mouse IgG using the developed ITS device. After 6-min competitive immunoreaction, the tracer antibody was captured on the test zone, which led to accumulation of LuReGNPs. The appearance of red color resulting from LuReGNPs accumulation on the test zone was observed by naked eyes as a qualitative and semi-quantitative signal. Moreover, luminophore in the signal probes enabled sensitive quantitation after initiating the luminol-H₂O₂ chemiluminescent reaction. Under the optimal conditions, mouse IgG was detected within a linear range of 0.10–100 ng/mL with a detection limit of 0.033 ng/mL (S/N = 3). The results for real biological samples detection and recovery tests demonstrated the acceptable reliability of the dual-response ITS. It exhibited great potential in both rapid screening and sensitive quantitation. Due to the merits of facile manipulation, short detection time and ideal portability, the platform could be further extended to multianalyte immunoassay of biomarker proteins for point-of-care test.

制备了减少鲁米诺的金纳米颗粒（LuReGNPs），并用作用于开发比色/化学发光免疫色谱测试条（ITS）的双反应信号探针。LuReGNPs标记的抗体被用作信号示踪剂，使用开发的ITS装置进行小鼠IgG的免疫测定。竞争性免疫反应6分钟后，示踪抗体被捕获在测试区，导致LuReGNP积累。肉眼观察到由于LuReGNPs积聚在测试区域而导致的红色外观，作为定性和半定量信号。此外，信号探针中的发光体在启动luminol-H ₂ O ₂后能够进行灵敏定量化学发光反应。在最佳条件下，小鼠IgG的线性范围为0.10–100 ng / mL，检出限为0.033 ng / mL（S / N = 3）。实际生物样品检测和回收测试的结果证明了双响应ITS的可接受的可靠性。它在快速筛选和灵敏定量分析中都显示出巨大的潜力。由于操作简便，检测时间短和理想的可移植性的优点，该平台可进一步扩展至用于现场护理测试的生物标志物蛋白质的多分析物免疫测定。