Delineating the basic cellular components of cortical inhibitory circuits remains a fundamental issue in order to understand their specific contributions to microcircuit function. It is still unclear how current classifications of cortical interneuron subtypes relate to biological processes such as their developmental specification. Here we identified the developmental trajectory of neurogliaform cells (NGCs), the main effectors of a powerful inhibitory motif recruited by long-range connections. Using in vivo genetic lineage-tracing in mice, we report that NGCs originate from a specific pool of 5-HT3AR-expressing Hmx3+ cells located in the preoptic area (POA). Hmx3-derived 5-HT3AR+ cortical interneurons (INs) expressed the transcription factors PROX1, NR2F2, the marker reelin but not VIP and exhibited the molecular, morphological and electrophysiological profile of NGCs. Overall, these results indicate that NGCs are a distinct class of INs with a unique developmental trajectory and open the possibility to study their specific functional contribution to cortical inhibitory microcircuit motifs.

中文翻译：

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神经胶质样皮质中间神经元来源于视前区的细胞

描绘皮质抑制电路的基本细胞成分仍然是一个基本问题，以了解它们对微电路功能的具体贡献。目前尚不清楚皮质中间神经元亚型的当前分类如何与生物过程（例如其发育规范）相关。在这里，我们确定了神经胶质细胞 (NGC) 的发育轨迹，神经胶质细胞是通过远程连接招募的强大抑制基序的主要效应物。在小鼠中使用体内遗传谱系追踪，我们报告说 NGC 源自位于视前区 (POA) 的特定的表达 5-HT3AR 的 Hmx3+ 细胞池。Hmx3 衍生的 5-HT3AR+ 皮质中间神经元 (INs) 表达转录因子 PROX1、NR2F2、标记物 reelin 但不表达 VIP，并表现出分子、NGCs 的形态学和电生理学特征。总体而言，这些结果表明 NGC 是一类独特的 IN，具有独特的发展轨迹，并为研究它们对皮质抑制微电路基序的特定功能贡献提供了可能性。