The absolute configuration of Stachybotrin C was confirmed in this study. After synthesizing the dimethyl ethers of Stachybotrin C, the C-8 epimer was analyzed by 1D NOESY. However, the stereochemistry determination was difficult through the NOE correlations. Instead, the di(4-bromobenzyl) ether of Stachybotrin C was derived and used for X-ray diffraction analysis, because its single crystal was easier to obtain than that of the original Stachybotrin C. The stereochemistry of Stachybotrin C was determined to be (8S, 9R). This derivatization approach may also be used to prepare single crystals of the analogues.

中文翻译：

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使用其4-溴苄基醚衍生物的单晶X射线衍射分析确定水苏蛋白C的绝对构型。

这项研究证实了水苏蛋白C的绝对构型。合成水飞蓟宾C的二甲醚后，通过一维NOESY分析C-8差向异构体。然而，通过NOE相关性难以确定立体化学。相反，因为其单晶比原始的Stachybotrin C更容易获得，所以获得了Stachybotrin C的二（4-溴苄基）醚并用于X射线衍射分析.Stachybotrin C的立体化学被确定为（ 8S，9R）。这种衍生化方法也可以用于制备类似物的单晶。