As the main hemicellulose of poplar, the interaction of xylan with lignin was expected to have profound effect on biomass recalcitrance. In this paper, the dynamic changes of xylan and lignin in poplar cell wall during a mild pretreatment using γ-valerolactone (GVL) was investigated using chemical and microscopic techniques. Synergetic dissolution of branched xylan and lignin from the secondary wall of the fiber cell was found to play a major role in opening the cell wall structure for enzymatic attack. In the case of the removal of xylan and lignin reaching a certain level, β-O-4′ cleavage of lignin which destroyed its interaction with hydrophobic cellulose face was found to make great contribution to the enhanced enzymatic hydrolysis. The deep understanding of this process could lead to a new insight into the understanding of the plant cell wall architecture and provide basic information for biomass processing.

中文翻译：

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分支木聚糖和木质素的协同溶解为杨树细胞壁的酶水解开辟了道路

作为杨树的主要半纤维素，木聚糖与木质素的相互作用有望对生物量的顽固性产生深远的影响。本文采用化学和显微技术研究了γ-戊内酯（GVL）温和预处理对杨树细胞壁中木聚糖和木质素的动态变化。发现支链木聚糖和木质素从纤维细胞的次生壁上的协同溶解在打开细胞壁结构进行酶攻击中起主要作用。在去除木聚糖和木质素达到一定水平的情况下，发现木质素的β-O-4'裂解破坏了其与疏水性纤维素表面的相互作用，为增强酶水解做出了巨大贡献。