Identification and Characterization of a Membrane-Bound Sesterterpene Cyclase from Streptomyces somaliensis,Journal of Natural Products

当前位置： X-MOL 学术 › J. Nat. Prod. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Identification and Characterization of a Membrane-Bound Sesterterpene Cyclase from Streptomyces somaliensis
Journal of Natural Products ( IF 3.3 ) Pub Date : 2018-03-19 00:00:00 , DOI: 10.1021/acs.jnatprod.7b01033
Yanlong Yang ₁ , Yuting Zhang _{1,

2} , Shasha Zhang ₃ , Qingwen Chen ₄ , Ke Ma _{1,

2} , Li Bao _{1,

2} , Yong Tao ₃ , Wenbing Yin _{1,

2} , Guodong Wang ₄ , Hongwei Liu _{1,

2}

Affiliation

Sesterterpenes are usually found in plants and fungi, but are rare in bacteria. Here, we present the identification of StsC from Streptomyces somaliensis, a member of the UbiA superfamily, as a membrane-bound sesterterpene cyclase in bacteria. The cyclized products for StsC, somaliensenes A (1) and B (2), were identified by expressing the corresponding gene in an engineered Escherichia coli strain. The structures of 1 and 2 were determined by analysis of the NMR and MS spectroscopic data.

中文翻译：

索马里链霉菌膜结合的酯基环戊二烯环化酶的鉴定与表征

酯基萜烯通常存在于植物和真菌中，但在细菌中却很少见。在这里，我们介绍了从UbiA超家族成员Somali链霉菌中StsC的鉴定，将其作为细菌中的膜结合型Sesterterpene环化酶。通过在工程化的大肠杆菌菌株中表达相应的基因，鉴定出StsC的环化产物，即索马里色菌素A（1）和B（2）。1和2的结构是通过NMR和MS光谱数据分析确定的。

更新日期：2018-03-19

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11