High performance liquid chromatography, coupled with a benchtop Q-Exactive Orbitrap high-resolution mass spectrometer, was successfully applied for the determination of 24 target antibiotics (selected beta-lactams, tetracyclines, fluoroquinolones, sulfonamids, phenicols, macrolides, cephalosporins, lincosamides, diaminopyrimidine) in fish matrices. The Q-Exactive parameters were carefully studied to accomplish the best compromise between a suitable scan speed and selectivity, considering the restrictions associated with generic sample preparation methodology. Retention time, an exact mass with tolerance of 2 ppm and data-dependent MS² spectra were the main identifiers. The method was validated through specificity, linearity, recovery, intra- and inter-day repeatability, decision limit (CCα) and detection capability (CCβ), according to 2002/657/EC. The values of CCα and CCβ ranged from 29.2 to 36.8 and 32.5 to 48.9, respectively, while overall recovery ranged from 91.1 to 105.6%. Fifty fish samples were analysed, showing the sporadic incidence of enrofloxacin, chlortetracycline, oxytetracycline, amoxicillin and trimethoprim, albeit below the maximum residual levels.

高效液相色谱仪与台式Q-Exactive Orbitrap高分辨率质谱仪相结合，已成功用于测定24种目标抗生素（选定的β-内酰胺，四环素，氟喹诺酮类，磺胺类，酚类，大环内酯类，头孢菌素，林可酰胺，二氨基嘧啶）在鱼基质中。考虑到通用样品制备方法的局限性，对Q-Exactive参数进行了仔细研究，以在合适的扫描速度和选择性之间实现最佳折衷。保留时间，精确的质量（公差为2 ppm）和取决于数据的MS ²光谱是主要的识别符。根据2002/657 / EC，该方法通过特异性，线性，回收率，日内和日间重复性，决策极限（CCα）和检测能力（CCβ）进行了验证。CCα和CCβ值的范围分别为29.2至36.8和32.5至48.9，而总回收率的范围为91.1至105.6％。分析了五十个鱼样品，显示了恩诺沙星，金霉素，土霉素，阿莫西林和甲氧苄氨辛酯的零星发生率，尽管低于最大残留水平。