We synthesized Ni²⁺-attached O-Carboxymethyl chitosan Schiff base complexes embedded composite cryogels (Ni²⁺-O-CMCS-CCs) by means of polymerization of gel-forming precursors at subzero temperatures. Prepared affinity cryogel showed excellent adsorption performance for lysozyme selected as model protein to test adsorption parameters, demonstrating an adsorption capacity of 244.6 mg/g (15.3 mg/g for Ni²⁺ minus O-CMCS-CCs), with fast adsorption equilibrium within 30 min and good reversibility. The performance of Ni²⁺-O-CMCS-CCs for lysozyme was also evaluated by SDS-PAGE, and a purification efficiency of 86.9% with 89.5% purification yield was determined. The swelling test, FT-IR, and SEM analysis were carried out for the characterization of Ni²⁺-O-CMCS-CCs. At the end of 35 adsorption-desorption cycles, there was no significant change in the adsorption capacity.

我们通过在零以下温度下形成凝胶的前体进行聚合，合成了附有Ni ^2+的邻羧甲基壳聚糖席夫碱配合物嵌入的复合冷冻凝胶（Ni ²⁺ -O-CMCS- CCs ）。制备的亲和力冷冻凝胶对被选作模型蛋白的溶菌酶表现出优异的吸附性能，以测试吸附参数，表现出244.6 mg / g的吸附能力（Ni ²⁺减去O-CMCS- CCs的吸附能力为15.3 mg / g ），在30内具有快速吸附平衡分钟和良好的可逆性。Ni ²⁺的性能还通过SDS-PAGE评估了溶菌酶的-O-CMCS-CC，并且确定了86.9％的纯化效率和89.5％的纯化产率。对Ni ²⁺ -O-CMCS-CCs进行了溶胀试验，FT-IR和SEM分析。在35个吸附-解吸循环结束时，吸附容量没有显着变化。