During pregnancy, exposure to environmental contaminants can lead to adverse effects on fetal growth and development, especially by targeting the placenta. Di(2-ethylhexyl)phthalate (DEHP), the most abundant chemical used in plastic materials, is known to induce toxicity on animals reproductive system and is suspected to give rise to similar effect in humans. Toxicity of DEHP is due to its main metabolite, MEHP, which is also known to disturb lipid synthesis in several organs. Moreover, mono-(2-ethylhexyl)phtalate (MEHP) is a high affinity ligand of the peroxisome proliferator-activated receptor PPARγ which is essential for placental development and lipid metabolism. In order to investigate possible lipid disruptions induced by MEHP, in the JEG-3 human trophoblast cell line, a differential lipidomic analysis was carried out by UPLC-MS on both exposed and control cells. Our results showed that MEHP induced an important change of JEG-3 cells lipidome, especially in glycerolipids and glycerophospholipids, with a marked accumulation of triacylglycerols. For the first time, our results highlighted adverse effects of MEHP on human placental cells lipidome and thus, its potential effect on placental physiology.

在怀孕期间，暴露于环境污染物会导致对胎儿生长和发育的不利影响，特别是通过靶向胎盘。众所周知，邻苯二甲酸二（2-乙基己基）酯（DEHP）是塑料材料中使用最丰富的化学物质，对动物的生殖系统具有毒性，并被怀疑对人类产生类似的作用。DEHP的毒性是由于它的主要代谢物MEHP引起的，MEHP还已知会干扰几个器官的脂质合成。此外，单（2-乙基己基）邻苯二甲酸酯（MEHP）是过氧化物酶体增殖物激活的受体PPARγ的高亲和力配体，它对胎盘发育和脂质代谢至关重要。为了研究MEHP诱导的可能的脂质破坏，在JEG-3人滋养层细胞系中，通过UPLC-MS对暴露的细胞和对照细胞进行差异脂质组分析。我们的结果表明，MEHP诱导了JEG-3细胞脂质组的重要变化，尤其是在甘油脂和甘油磷脂中，三酰基甘油的积累显着。我们的研究结果首次突显了MEHP对人胎盘细胞脂质组的不利影响，并因此突出了其对胎盘生理的潜在影响。