This paper presents a novel microphotonic sensor based on silicon technologies for high-throughput single cell measurements. It employs a highly sensitive Fabry-Pérot resonant cavity to extract cellular refractive index information. The integrated large cross-section rib waveguides provide a single-mode like behavior important for resonant cell sensing. Differentiated myeloid cells derived from a promyelocytic leukemia cell line were injected in a microchannel, sheathlessly focused using inertial forces and analyzed while flowing through the resonant cavity volume. Results were compared against a commercial flow cytometer and showed a substantial improvement on cellular discrimination. Thus, this sensor has the ability to discriminate cell populations, usually identified using fluorescent parameters, without any dyes and can reach measurement rate as high as 2000 cells per second. By harnessing the cell's effective volume refractive index, our device offers complementary measurements readily improving actual technologies and thus providing crucial information for research and clinical professionals.

本文提出了一种基于硅技术的新型微光子传感器，用于高通量单细胞测量。它采用高度敏感的Fabry-Pérot谐振腔来提取细胞折射率信息。集成的大截面肋形波导提供了对谐振单元感应非常重要的类似单模的行为。将源自早幼粒细胞白血病细胞系的分化髓样细胞注射到微通道中，使用惯性力无鞘聚焦，并在流过共振腔体积的同时进行分析。将结果与商用流式细胞仪进行了比较，显示出细胞分辨力的显着改善。因此，此传感器具有区分通常使用荧光参数识别的细胞群体的能力，无需任何染料，测量速率可高达每秒2000个细胞。通过利用细胞的有效体积折射率，我们的设备可以提供互补的测量结果，从而轻松地改善实际技术，从而为研究和临床专业人员提供重要信息。