This work reports on a simple method for the determination of lysine content by an in situ sample pretreatment and headspace gas chromatographic measurement (HS-GC) technique, based on carbon dioxide (CO₂) formation from the pretreatment reaction (between lysine and ninhydrin solution) in a closed vial. It was observed that complete lysine conversion to CO₂ could be achieved within 60 min at 60 °C in a phosphate buffer medium (pH = 4.0), with a minimum molar ratio of ninhydrin/lysine of 16. The results showed that the method had a good precision (RSD < 5.23%) and accuracy (within 6.80%), compared to the results measured by a reference method (ninhydrin spectroscopic method). Due to the feature of in situ sample pretreatment and headspace measurement, the present method becomes very simple and particularly suitable to be used for batch sample analysis in lysine-related research and applications.

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上的赖氨酸含量的测定的简单方法原位样品预处理和顶部空间气相色谱测定（HS-GC）技术的基础上，二氧化碳这项工作报告由（CO ₂）从预处理反应（赖氨酸和茚三酮溶液之间形成）放在封闭的小瓶中。观察到赖氨酸完全转化为CO ₂可以在60°C的磷酸盐缓冲液（pH = 4.0）中于60分钟内完成，茚三酮/赖氨酸的最小摩尔比为16。结果表明该方法具有良好的精密度（RSD <5.23％）和与参考方法（茚三酮光谱法）测得的结果相比，准确度（6.80％以内）。由于原位样品预处理和顶空测量的特征，本发明的方法变得非常简单，特别适合用于赖氨酸相关研究和应用中的批量样品分析。

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