Mass Spectrometry Approaches to Glycomic and Glycoproteomic Analyses,Chemical Reviews

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Mass Spectrometry Approaches to Glycomic and Glycoproteomic Analyses
Chemical Reviews ( IF 51.4 ) Pub Date : 2018-03-19 00:00:00 , DOI: 10.1021/acs.chemrev.7b00732
L Renee Ruhaak ₁ , Gege Xu ₂ , Qiongyu Li ₂ , Elisha Goonatilleke ₂ , Carlito B Lebrilla _{2,

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Affiliation

Glycomic and glycoproteomic analyses involve the characterization of oligosaccharides (glycans) conjugated to proteins. Glycans are produced through a complicated nontemplate driven process involving the competition of enzymes that extend the nascent chain. The large diversity of structures, the variations in polarity of the individual saccharide residues, and the poor ionization efficiencies of glycans all conspire to make the analysis arguably much more difficult than any other biopolymer. Furthermore, the large number of glycoforms associated with a specific protein site makes it more difficult to characterize than any post-translational modification. Nonetheless, there have been significant progress, and advanced separation and mass spectrometry methods have been at its center and the main reason for the progress. While glycomic and glycoproteomic analyses are still typically available only through highly specialized laboratories, new software and workflow is making it more accessible. This review focuses on the role of mass spectrometry and separation methods in advancing glycomic and glycoproteomic analyses. It describes the current state of the field and progress toward making it more available to the larger scientific community.

中文翻译：

糖组学和糖蛋白质组学分析的质谱方法

糖组学和糖蛋白质组学分析涉及与蛋白质缀合的寡糖（聚糖）的表征。聚糖是通过复杂的非模板驱动过程产生的，涉及延伸新生链的酶的竞争。结构的巨大多样性、单个糖残基极性的变化以及聚糖的电离效率差，所有这些都使得分析可能比任何其他生物聚合物困难得多。此外，与特定蛋白质位点相关的大量糖型使其比任何翻译后修饰都更难以表征。尽管如此，还是取得了重大进展，先进的分离和质谱方法一直是其核心，也是取得进展的主要原因。虽然糖组学和糖蛋白质组学分析通常仍然只能通过高度专业化的实验室进行，但新的软件和工作流程使其更容易实现。本综述重点关注质谱和分离方法在推进糖组学和糖蛋白质组学分析中的作用。它描述了该领域的现状以及使其更容易被更大的科学界所利用的进展。

更新日期：2018-03-19

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