Carbohydrate Polymers ( IF 10.7 ) Pub Date : 2018-03-19 , DOI: 10.1016/j.carbpol.2018.03.044 Raquel Gallardo-Rivera , María de los Ángeles Aguilar-Santamaría , Phaedra Silva-Bermúdez , Julieta García-López , Alberto Tecante , Cristina Velasquillo , Angélica Román-Guerrero , César Pérez-Alonso , Humberto Vázquez-Torres , Keiko Shirai
Chitosan, sodium alginate and gel of Aloe vera (Aloe barbadensis Miller) were employed for the preparation of polyelectrolyte complexes at pH 4 and 6. FT-IR spectroscopy analysis showed evidence on complexes formation and incorporation of the Aloe vera gel. The ζ potential determination of the polyelectrolyte complexes revealed the presence of surface charges in the range of −20 to −24 mV, which results in stable systems. The dynamic moduli exhibited a high dependence on angular frequency, which is commonly found in solutions of macromolecules. The materials showed human fibroblast and lymphocyte viabilities up to 90% in agreement with null cytotoxicity. The polyelectrolyte complexes at pH 6 with Ca2+ were stable, showed high water absorption, satisfactory morphology, pore size and rigidity, characteristics that allowed significant human fibroblast migration in wound closure in vitro assays.
中文翻译:
芦荟,壳聚糖和藻酸盐的聚电解质复合物可产生成纤维细胞和淋巴细胞活力并迁移
用壳聚糖,藻酸钠和芦荟凝胶(芦荟米勒)在pH 4和6下制备聚电解质复合物。FT-IR光谱分析显示了芦荟凝胶形成复合物和结合的证据。聚电解质配合物的ζ电位测定表明,存在表面电荷的范围为-20至-24 mV,这导致系统稳定。动态模量表现出对角频率的高度依赖性,这在大分子溶液中很常见。这些材料显示出人类成纤维细胞和淋巴细胞活力高达90%,与无细胞毒性一致。pH 6与Ca 2+的聚电解质复合物稳定,显示出高吸水率,令人满意的形态,孔径和刚度,这些特性使人成纤维细胞在伤口闭合体外试验中具有明显的迁移能力。