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Development and application of a portable fluorescence detector for shipboard analysis of ammonium in estuarine and coastal waters†
Analytical Methods ( IF 2.7 ) Pub Date : 2018-03-19 00:00:00 , DOI: 10.1039/c8ay00438b Yong Zhu 1, 2, 3, 4, 5 , Jianfang Chen 1, 2, 3, 4, 5 , Xiaolai Shi 1, 2, 3, 4, 5 , Dongxing Yuan 6, 7, 8, 9, 10 , Sichao Feng 10, 11, 12, 13, 14 , Tingjin Zhou 10, 15, 16 , Yongming Huang 6, 7, 8, 9, 10
Analytical Methods ( IF 2.7 ) Pub Date : 2018-03-19 00:00:00 , DOI: 10.1039/c8ay00438b Yong Zhu 1, 2, 3, 4, 5 , Jianfang Chen 1, 2, 3, 4, 5 , Xiaolai Shi 1, 2, 3, 4, 5 , Dongxing Yuan 6, 7, 8, 9, 10 , Sichao Feng 10, 11, 12, 13, 14 , Tingjin Zhou 10, 15, 16 , Yongming Huang 6, 7, 8, 9, 10
Affiliation
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Herein, a novel homemade portable fluorescence detector that is simple, smaller, and transportable for the shipboard analysis of ammonium in estuarine and coastal waters has been developed and applied in a flow system for ammonium analysis based on the reaction of ammonium, OPA, and sulfite. The detector incorporates a UV-LED, two band pass filters, a photomultiplier tube (PMT), a modified flow cell, and an electronic circuit with a constant voltage supply and a constant current supply. The modified flow cell of the detector used in this study can avoid the interference of air bubbles in the fluorescence signal. A 100 nmol L−1 ammonium standard solution was continuously measured for 90 min. An RSD of 0.8% (n = 38) was obtained, indicating the good stability of the detector. Compared with the commercial PMT-FL fluorescence detector, the homemade detector was smaller and lightweight and demonstrated better sensitivity (approximately 11.4% higher). Moreover, the parameters of the flow system were optimized. Under optimal conditions, a detection limit of 2.1 nmol L−1 within the working range of 0–300 nmol L−1 was obtained. Using the underway sampling system, the homemade portable fluorescence detector was applied in Jiulongjiang estuary, and the distribution of ammonium in the surface seawater was obtained.
中文翻译：
便携式船用荧光检测器的开发和应用，用于船上河口和沿海水体中的氨分析†
在此，已经开发出一种新颖的，便携式的，便携式的荧光检测器，该检测器简单，体积小并且可运输，用于船上和河口水中的铵的分析，并且基于铵，OPA和亚硫酸盐的反应，将其应用在用于铵分析的流动系统中。 。该检测器包含一个UV-LED，两个带通滤光片，一个光电倍增管（PMT），一个改进的流通池以及一个带有恒定电压源和恒定电流源的电子电路。本研究中使用的检测器的改进型流通池可以避免气泡干扰荧光信号。连续测量100 nmol L -1铵标准溶液90分钟。相对标准偏差为0.8％（n= 38），表明检测器具有良好的稳定性。与商用PMT-FL荧光检测器相比，自制检测器体积更小，重量更轻且灵敏度更高（约高11.4％）。此外，优化了流动系统的参数。在最佳条件下，在0-300 nmol L -1的工作范围内获得了2.1 nmol L -1的检出限。利用正在进行的采样系统，将自制的便携式荧光检测器应用于九龙江口，获得了铵盐在地表海水中的分布。
更新日期：2018-03-19
中文翻译：
便携式船用荧光检测器的开发和应用，用于船上河口和沿海水体中的氨分析†
在此，已经开发出一种新颖的，便携式的，便携式的荧光检测器，该检测器简单，体积小并且可运输，用于船上和河口水中的铵的分析，并且基于铵，OPA和亚硫酸盐的反应，将其应用在用于铵分析的流动系统中。 。该检测器包含一个UV-LED，两个带通滤光片，一个光电倍增管（PMT），一个改进的流通池以及一个带有恒定电压源和恒定电流源的电子电路。本研究中使用的检测器的改进型流通池可以避免气泡干扰荧光信号。连续测量100 nmol L -1铵标准溶液90分钟。相对标准偏差为0.8％（n= 38），表明检测器具有良好的稳定性。与商用PMT-FL荧光检测器相比，自制检测器体积更小，重量更轻且灵敏度更高（约高11.4％）。此外，优化了流动系统的参数。在最佳条件下，在0-300 nmol L -1的工作范围内获得了2.1 nmol L -1的检出限。利用正在进行的采样系统，将自制的便携式荧光检测器应用于九龙江口，获得了铵盐在地表海水中的分布。
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