Abstract In this work a porous collagen/hydroxyapatite matrices were fabricated by the freeze-drying method and then modified using a dehydrothermal treatment. The aim of present study was to investigate the biological properties of prepared scaffolds. Both in vitro as well as in vivo methods were used for these studies. The in vitro study were performed using mouse fibroblasts cells line NIH 3T3 and in vivo by subdermal and peritoneal cavity implantation in rats for up 30 days. The results of in vitro cytotoxity tests indicated that the samples were well tolerated by the cells. Good biocompatibility is demonstrated by the absence of necrosis or abscess formation in the surrounding tissues. This study provides valuable insight, which may lead to new applications of collagen/hydroxyapatite matrices in the field of tissue engineering.

中文翻译：

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改性胶原蛋白/羟基磷灰石复合材料的体外和体内初步评估

摘要 在这项工作中，通过冷冻干燥方法制备了多孔胶原/羟基磷灰石基质，然后使用脱水热处理进行改性。本研究的目的是研究制备的支架的生物学特性。体外和体内方法都用于这些研究。体外研究使用小鼠成纤维细胞系 NIH 3T3 进行，并通过皮下和腹膜腔植入大鼠体内进行长达 30 天。体外细胞毒性试验结果表明样品对细胞具有良好的耐受性。周围组织没有坏死或脓肿形成，证明了良好的生物相容性。这项研究提供了有价值的见解，可能会导致胶原蛋白/羟基磷灰石基质在组织工程领域的新应用。