In this study, silver nanoparticles modified choline chloride functionalized graphene oxide (AgNPs-ChCl-GO) was synthesized using sonochemical method and utilized as a bioelectrochemical sensor for detection of celecoxibe (CEL). The characterization studies were ultimately performed in order to acheive a more complete understanding of the morphological and structural features of the AgNPs-ChCl-GO using different techniques including FT-IR, AFM, FE-SEM, EDX, and XRD. AgNPs-ChCl-GO demonstrated a significant improvement in the reduction activity of CEL due to the enhancement in the current response compared to the bare carbon paste electrode (CPE). The optimum experimental conditions, were optimized using central composite design (CCD) methodology. The differential pulse voltammetry (DPVs) showed an expanded linear dynamic ranges of 9.6 × 10^-9- 7.4 × 10^-7 M for celecoxib in Britton-Robinson buffer in pH 5.0 with. LOD (S/N = 3) and LOQ (S/N = 10) were obtained 2.51 × 10^-9 M and 6.58 × 10^-9 M respectively. AgNPs-ChCl-GO-carbon paste electrode exhibited suitable properties and high accuracy determination of celecoxib in the human plasma sample.

在这项研究中，使用声化学方法合成了纳米银修饰的氯化胆碱官能化的氧化石墨烯银（AgNPs-ChCl-GO），并将其用作生物化学传感器来检测塞来昔布（CEL）。最终进行了表征研究，以使用不同的技术（包括FT-IR，AFM，FE-SEM，EDX和XRD）更全面地了解AgNPs-ChCl-GO的形态和结构特征。与裸碳糊电极（CPE）相比，由于电流响应的增强，AgNPs-ChCl-GO证明了CEL还原活性的显着改善。使用中央复合设计（CCD）方法优化了最佳实验条件。差分脉冲伏安法（DPV）显示了9.6×10的扩展线性动态范围^-9 - 7.4×10 ^-7 M代表在的BR塞来昔布在缓冲pH值为5.0。LOD（S / N = 3）和LOQ（S / N = 10）分别为2.51×10 ^-9 M和6.58×10 ^-9M。AgNPs-ChCl-GO-碳糊电极表现出合适的性能，并能高精度测定人血浆样品中的塞来昔布。