The development of a fluorescent multiplexed microarray platform able to detect and quantify a wide variety of pollutants in seawater is reported. The microarray platform has been manufactured by spotting 6 different bioconjugate competitors and it uses a cocktail of 6 monoclonal or polyclonal antibodies raised against important families of chemical pollutants such as triazine biocide (i.e. Irgarol 1051®), sulfonamide and chloramphenicol antibiotics, polybrominated diphenyl ether flame-retardant (PBDE, i.e. BDE-47), hormone (17β-estradiol), and algae toxin (domoic acid). These contaminants were selected as model analytes, however, the platform developed has the potential to detect a broader group of compounds based on the cross-reactivity of the immunoreagents used. The microarray chip is able to simultaneously determine these families of contaminants directly in seawater samples reaching limits of detection close to the levels found in contaminated areas (Irgarol 1051®, 0.19 ± 0,06 µg L^-1; sulfapyridine, 0.17 ± 0.07 µg L^-1; chloramphenicol, 0.11 ± 0.03 µg L^-1; BDE-47, 2.71 ± 1.13 µg L^-1; 17β-estradiol, 0.94 ± 0.30 µg L^-1 and domoic acid, 1.71 ± 0.30 µg L^-1). Performance of the multiplexed microarray chip was assessed by measuring 38 blind spiked seawater samples containing either one of these contaminants or mixtures of them. The accuracy found was very good and the coefficient of variation was < 20% in all the cases. No sample pre-treatment was necessary, and the results could be obtained in just 1 h 30 min. The microarray shows high sample throughput capabilities, being able to measure simultaneously more than 68 samples and screen them for a significant number of chemical contaminants of interest in environmental screening programs.

据报道，荧光多路复用微阵列平台的开发能够检测和量化海水中的多种污染物。该微阵列平台是通过发现6种不同的生物共轭物竞争者而制造的，它使用6种针对重要化学污染物（例如三嗪杀生物剂（即Irgarol1051®），磺酰胺和氯霉素抗生素，多溴联苯醚火焰）的单克隆或多克隆抗体组成的混合物。 -缓释剂（PBDE，即BDE-47），激素（17β-雌二醇）和藻类毒素（十二酸）。选择了这些污染物作为模型分析物，但是，开发的平台具有潜力，可以根据使用的免疫试剂的交叉反应性检测更广泛的化合物。^-1 ; 磺胺吡啶，0.17±0.07μgL ^-1 ; 氯霉素，0.11±0.03 µg L ^-1；BDE-47，2.71±1.13 µg L ^-1；17β-雌二醇，0.94±0.30 µg L ^-1和多摩酸，1.71±0.30 µg L ^-1）。通过测量38个包含这些污染物之一或它们的混合物的盲掺海水样品，可以评估多路复用微阵列芯片的性能。在所有情况下，发现的准确性都非常好，并且变异系数<20％。无需样品预处理，只需1小时30分钟即可获得结果。该微阵列显示出高的样品通量能力，能够同时测量68个以上的样品并针对环境筛选程序中的大量感兴趣的化学污染物进行筛选。