The purpose of this study was to investigate the anaerobic digestion (AD) of rice straw at different temperatures in a 300 m³ bioreactor. The results showed that the biogas yield was 401.9 m³/ton (dry straw weight) in this AD system. The contents of total solids, volatile solids, chemical oxygen demand, pH, NH₄⁺-N, and volatile fatty acids were all in the optimal range, indicating that the entire AD system was stable and efficient. In addition, the phylum Bacteroidetes was the main type of bacteria found during the AD process. When the temperature increased, the relative abundance ratio of Methanoculleus, Methanosarcina, and Firmicutes increased. The phyla Bacteroidetes and Fibrobacteres predominated in straw samples, and the relative abundance ratio of Firmicutes and Bacteroidetes in thermophilic AD was much greater than that in mesophilic AD. This study provides new evidence regarding the effects of temperature on the community changes of specific microbiota in the AD of rice straw.

中文翻译：

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39–50°C稻草厌氧消化过程中的沼气生产和微生物群落动态：一项初步研究

这项研究的目的是研究在300 m ³生物反应器中不同温度下稻草的厌氧消化（AD）。结果表明，在该AD系统中，沼气产量为401.9 m ³ /吨（秸秆干重）。总固形物，挥发性固形物，化学需氧量，pH，NH ₄ ⁺ -N和挥发性脂肪酸的含量均在最佳范围内，表明整个AD系统是稳定有效的。另外，门生细菌是AD过程中发现的主要细菌类型。当温度升高时，甲烷菌，甲烷菌的相对丰度比，并且Firmicutes增加了。秸秆样品中的细菌类杆菌和纤维杆菌居多，嗜热性AD中菌类和拟杆菌的相对丰度比要大得多。这项研究提供了有关温度对稻草AD中特定微生物群落变化的影响的新证据。