Abstract The hydrophobins (HFB)s are a class of proteins with high surface tension and thermostability which potential application in enhanced oil recovery (EOR). The entomopathogenic fungus Lecanicillium lecanii produced HFBs in an 8.8L-scale solid-state fermentation using polyurethane foams as inert support in three configurations. Additionally, crude enzyme with lipase activity was also produced and tested for EOR individually or in combination with HFBs. The polyurethane foam support in cubes attained the highest yield of class I HFB (17.3 ± 1%) and the highest lipase activity (3.6 ± 0.2 U/mg protein). These HFBs formed stable oil–water emulsions displaying an interfacial tension up to 7.6 ± 0.3 mN/m. The proteins were used in limestone cores under tertiary recovery oil well conditions to achieve up to 14% recovery.

中文翻译：

---

来自 Lecanicillium lecanii 的疏水蛋白提高石油采收率

摘要 疏水蛋白（HFB）是一类具有高表面张力和热稳定性的蛋白质，在提高采收率（EOR）方面具有潜在的应用前景。昆虫病原真菌 Lecanicillium lecanii 在 8.8 L 规模的固态发酵中使用聚氨酯泡沫作为三种配置的惰性载体产生 HFB。此外，还生产了具有脂肪酶活性的粗酶，并单独或与 HFB 组合测试了 EOR。立方体中的聚氨酯泡沫载体获得了最高的 I 类 HFB 产率 (17.3 ± 1%) 和最高的脂肪酶活性 (3.6 ± 0.2 U/mg 蛋白质)。这些 HFB 形成稳定的油水乳液，界面张力高达 7.6 ± 0.3 mN/m。在三次采收油井条件下，将蛋白质用于石灰岩岩心，以实现高达 14% 的采收率。