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Discriminant analysis of principal components and pedigree assessment of genetic diversity and population structure in a tetraploid potato panel using SNPs
PLOS ONE ( IF 2.9 ) Pub Date : 2018-03-16 , DOI: 10.1371/journal.pone.0194398
Sofía I Deperi 1 , Martín E Tagliotti 1 , M Cecilia Bedogni 2 , Norma C Manrique-Carpintero 3 , Joseph Coombs 3 , Ruofang Zhang 4 , David Douches 3 , Marcelo A Huarte 2
Affiliation  

The reported narrow genetic base of cultivated potato (Solanum tuberosum) can be expanded by the introgression of many related species with large genetic diversity. The analysis of the genetic structure of a potato population is important to broaden the genetic base of breeding programs by the identification of different genetic pools. A panel composed by 231 diverse genotypes was characterized using single nucleotide polymorphism (SNP) markers of the Illumina Infinium Potato SNP Array V2 to identify population structure and assess genetic diversity using discriminant analysis of principal components (DAPC) and pedigree analysis. Results revealed the presence of five clusters within the populations differentiated principally by ploidy, taxonomy, origin and breeding program. The information obtained in this work could be readily used as a guide for parental introduction in new breeding programs that want to maximize variability by combination of contrasting variability sources such as those presented here.



中文翻译:


使用 SNP 对四倍体马铃薯面板的主成分判别分析以及遗传多样性和种群结构的系谱评估



已报道的栽培马铃薯( Solanum tuberosum )狭窄的遗传基础可以通过许多具有大量遗传多样性的相关物种的渗入来扩大。马铃薯群体遗传结构的分析对于通过鉴定不同遗传库来拓宽育种计划的遗传基础非常重要。使用 Illumina Infinium Potato SNP Array V2 的单核苷酸多态性 (SNP) 标记对由 231 个不同基因型组成的小组进行表征,以识别群体结构并使用主成分判别分析 (DAPC) 和谱系分析评估遗传多样性。结果显示,种群内存在五个簇,主要通过倍性、分类学、起源和育种计划进行区分。这项工作中获得的信息可以很容易地用作新育种计划中亲本引入的指南,这些计划希望通过组合对比变异源(例如此处介绍的变异源)来最大化变异性。

更新日期:2018-03-17
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