In view of the report on anti-nociceptive activity of Leathery Murdah, Terminalia coriacea {Roxb.} Wight & Arn. (Combretaceae) leaves, the present study was conducted to isolate the active constituents and identify the underlying mechanisms. The methanolic extract of T. coriacea leaves (TCLME) at doses 125, 250 and 500 mg/kg orally, was subjected to various in-vivo assays in acetic acid induced writhing and formalin induced paw-licking tests with aspirin (100 mg/kg) and morphine (5 mg/kg) as reference drugs. Three flavonoids, rutin, robinin and gossypetin 3-glucuronide 8-glucoside were isolated and characterized from TCLME for the first time. The extract showed significant (p < 0.001) dose-dependent anti-nociceptive activity in glutamate induced paw licking in mice. The involvement of opioid pathway was confirmed as naloxone (5 mg/kg, i.p) treatment blocked the analgesic activity of the test extract. Similarly, glibenclamide (an ATP - sensitive potassium channel inhibitor) at dose of 10 mg/kg, i.p increased writhing in acetic acid model. It reversed the inhibitory effects of TCLME when administered in combination. Treatment of TCLME alone and in combination with l-arginine (100 mg/kg, i.p) significantly (p < 0.001) reduced writhing while pre-treatment with l-NAME (20 mg/kg, i.p) further enhanced the analgesic action of TCLME indicating involvement of nitric oxide pathway.

鉴于有关皮革化的默达（Terminalia coriacea）（Roxb。} Wight＆Arn。的抗伤害感受活性的报告。（Combretaceae）叶，进行本研究以分离活性成分并确定潜在机制。的甲醇提取物T.龟叶在剂量125,250和500（TCLME）毫克/公斤口服，进行各种的体内以阿司匹林（100毫克/千克）和吗啡（5毫克/千克）为参比药物，在乙酸诱导的扭体和福尔马林引起的舔爪试验中进行检测。首次从TCLME中分离并鉴定了三种类黄酮，芦丁，知更鸟和棉酚3-葡萄糖醛酸苷8-葡糖苷。该提取物在谷氨酸诱导的小鼠舔足中显示出显着的（p <0.001）剂量依赖性抗伤害感受活性。阿片类药物通路的参与被确认为纳洛酮（5 mg / kg，腹膜内）治疗阻断了测试提取物的镇痛活性。同样，在乙酸模型中，腹腔注射格列本脲（一种ATP敏感性钾通道抑制剂）的剂量为10 mg / kg，腹腔注射会增加扭体。组合使用时，它可以逆转TCLME的抑制作用。单独或与组合TCLME治疗升-精氨酸（100 mg / kg，ip）显着（p <0.001）减少了扭动，而用1 - NAME（20 mg / kg，ip）的预处理进一步增强了TCLME的镇痛作用，表明一氧化氮途径的参与。