Abstract Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus are the most notorious fish borne-pathogens responsible for several deadly diseases in human. However, the prevalence of these three pathogenic vibrios in Malaysian fishes has not been thoroughly investigated, leaving a study gap for the tracing of possible Vibrio outbreak through carrier fish consumption. The most-probable-number-PCR (MPN-PCR) assays are highly sensitive for the identification of a single bacterial pathogen but they have not been applied for the discrimination of major vibrios in a multiplex platform. This study coupled MPN method with Multiplex PCR to differentiate three major vibrios in a one test platform. A total of 120 fish samples were analyzed and the prevalence of V. parahaemolyticus, V. cholerae, and V. vulnificus was 48.33%, 27%, and 32.50%, respectively. When simplex MPN-PCR was applied similar results were obtained at P

中文翻译：

---

马来西亚生鱼中三种主要弧菌的多重 MPN-PCR

摘要 副溶血性弧菌、霍乱弧菌和创伤弧菌是最臭名昭著的鱼源性病原体，可导致多种人类致命疾病。然而，这三种致病性弧菌在马来西亚鱼类中的流行情况尚未得到彻底调查，这为通过食用载体鱼追踪可能的弧菌爆发留下了研究空白。最可能数 PCR (MPN-PCR) 检测对单一细菌病原体的鉴定高度敏感，但尚未应用于多重平台中主要弧菌的鉴别。本研究将 MPN 方法与多重 PCR 相结合，以在一个测试平台中区分三种主要的弧菌。共分析了 120 条鱼样本，副溶血性弧菌、霍乱弧菌和创伤弧菌的患病率分别为 48.33%、27% 和 32.50%，分别。当应用单纯 MPN-PCR 时，在 P 处获得了类似的结果