当前位置: X-MOL 学术Food Control › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Multiplex MPN-PCR for the enumeration of three major Vibrios in raw fishes in Malaysia
Food Control ( IF 5.6 ) Pub Date : 2018-08-01 , DOI: 10.1016/j.foodcont.2018.02.034
Sharmin Quazi Bonny , M.A. Motalib Hossain , Thong Kwai Lin , Md Eaqub Ali

Abstract Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus are the most notorious fish borne-pathogens responsible for several deadly diseases in human. However, the prevalence of these three pathogenic vibrios in Malaysian fishes has not been thoroughly investigated, leaving a study gap for the tracing of possible Vibrio outbreak through carrier fish consumption. The most-probable-number-PCR (MPN-PCR) assays are highly sensitive for the identification of a single bacterial pathogen but they have not been applied for the discrimination of major vibrios in a multiplex platform. This study coupled MPN method with Multiplex PCR to differentiate three major vibrios in a one test platform. A total of 120 fish samples were analyzed and the prevalence of V. parahaemolyticus, V. cholerae, and V. vulnificus was 48.33%, 27%, and 32.50%, respectively. When simplex MPN-PCR was applied similar results were obtained at P

中文翻译:

马来西亚生鱼中三种主要弧菌的多重 MPN-PCR

摘要 副溶血性弧菌、霍乱弧菌和创伤弧菌是最臭名昭著的鱼源性病原体,可导致多种人类致命疾病。然而,这三种致病性弧菌在马来西亚鱼类中的流行情况尚未得到彻底调查,这为通过食用载体鱼追踪可能的弧菌爆发留下了研究空白。最可能数 PCR (MPN-PCR) 检测对单一细菌病原体的鉴定高度敏感,但尚未应用于多重平台中主要弧菌的鉴别。本研究将 MPN 方法与多重 PCR 相结合,以在一个测试平台中区分三种主要的弧菌。共分析了 120 条鱼样本,副溶血性弧菌、霍乱弧菌和创伤弧菌的患病率分别为 48.33%、27% 和 32.50%,分别。当应用单纯 MPN-PCR 时,在 P 处获得了类似的结果
更新日期:2018-08-01
down
wechat
bug