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Beneficial effects of folic acid on the kidneys and testes of adult albino rats after exposure to methomyl†
Toxicology Research ( IF 2.2 ) Pub Date : 2018-03-15 00:00:00 , DOI: 10.1039/c7tx00309a Samar Sakr 1, 2, 3, 4 , Hanan Hassanien 1, 2, 3, 4 , Megan Jean Bester 5, 6, 7, 8 , Sandra Arbi 5, 6, 7, 8 , Azza Sobhy 1, 2, 3, 4 , Heba el Negris 2, 3, 4, 9, 10 , Vanessa Steenkamp 6, 7, 8, 11
Toxicology Research ( IF 2.2 ) Pub Date : 2018-03-15 00:00:00 , DOI: 10.1039/c7tx00309a Samar Sakr 1, 2, 3, 4 , Hanan Hassanien 1, 2, 3, 4 , Megan Jean Bester 5, 6, 7, 8 , Sandra Arbi 5, 6, 7, 8 , Azza Sobhy 1, 2, 3, 4 , Heba el Negris 2, 3, 4, 9, 10 , Vanessa Steenkamp 6, 7, 8, 11
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The aim of this study was to evaluate the protective effect of folate against methomyl-induced toxicity on the kidneys and testes of male rats. Adult male albino rats were divided into four groups; Group I served as the control (vehicle), Group II received folic acid (1.1 mg per kg b.wt.), Group III methomyl (1 mg per kg b.wt.) and Group IV folic acid and methomyl. Treatments were administered via oral gavage on a daily basis for 14 weeks. Thereafter blood samples were collected and serum creatinine, testosterone and total antioxidant capacity (TAC) were determined. Animals were sacrificed and semen analysis was conducted. The kidneys and testes were excised and malondialdehyde (MDA) levels were determined. Histopathological and immunohistochemical analyses for caspase-3 were also undertaken. Methomyl treatment resulted in a significant (p < 0.001) elevation of creatinine and MDA levels and significant (p < 0.001) reduction in testosterone and TAC levels. Furthermore, methomyl caused a significant (p < 0.001) reduction in sperm quality. Histopathological examination indicated testicular and renal damage with strong immunoreactivity for caspase-3. Functional and tissue damage was prevented in rats treated with a combination of methomyl and folic acid. This is ascribed to the ability of folate to directly scavenge reactive oxygen species and indirectly enhance cellular redox homeostasis. This study identified that folic acid supplementation may have a beneficial effect in preventing or reducing the deleterious effects of methomyl exposure on kidney as well as testis structure and function. Future studies should focus on the fertility outcome/pregnancy index in rats.
中文翻译：
叶酸暴露于灭多威后对成年白化病大鼠肾脏和睾丸的有益作用†
这项研究的目的是评估叶酸对灭多威对雄性大鼠肾脏和睾丸的毒性的保护作用。将成年雄性白化病大鼠分为四组。第一组作为对照（媒介物），第二组接受叶酸（1.1 mg / kg b.wt.），第三组甲磺酰（1 mg / kg b.wt.）和第四组叶酸和甲磺酰。通过以下方式进行治疗每天进行14天的管饲。此后，采集血样并测定血清肌酐，睾丸激素和总抗氧化能力（TAC）。处死动物并进行精液分析。切除肾脏和睾丸，并测定丙二醛（MDA）水平。还进行了caspase-3的组织病理学和免疫组化分析。灭多威处理导致显著（p肌酐和MDA水平和显著（的<0.001）高程p睾酮和TAC水平<0.001）减少。此外，灭多虫引起显著（p<0.001）降低精子质量。组织病理学检查表明睾丸和肾损伤对caspase-3具有强免疫反应性。用灭多威和叶酸治疗的大鼠可预防功能和组织损伤。这归因于叶酸直接清除活性氧和间接增强细胞氧化还原稳态的能力。这项研究表明，补充叶酸可能在预防或减少甲基苯丙胺暴露对肾脏以及睾丸结构和功能的有害影响方面具有有益作用。未来的研究应集中于大鼠的生育结局/妊娠指数。
更新日期：2018-03-15
中文翻译：
叶酸暴露于灭多威后对成年白化病大鼠肾脏和睾丸的有益作用†
这项研究的目的是评估叶酸对灭多威对雄性大鼠肾脏和睾丸的毒性的保护作用。将成年雄性白化病大鼠分为四组。第一组作为对照（媒介物），第二组接受叶酸（1.1 mg / kg b.wt.），第三组甲磺酰（1 mg / kg b.wt.）和第四组叶酸和甲磺酰。通过以下方式进行治疗每天进行14天的管饲。此后，采集血样并测定血清肌酐，睾丸激素和总抗氧化能力（TAC）。处死动物并进行精液分析。切除肾脏和睾丸，并测定丙二醛（MDA）水平。还进行了caspase-3的组织病理学和免疫组化分析。灭多威处理导致显著（p肌酐和MDA水平和显著（的<0.001）高程p睾酮和TAC水平<0.001）减少。此外，灭多虫引起显著（p<0.001）降低精子质量。组织病理学检查表明睾丸和肾损伤对caspase-3具有强免疫反应性。用灭多威和叶酸治疗的大鼠可预防功能和组织损伤。这归因于叶酸直接清除活性氧和间接增强细胞氧化还原稳态的能力。这项研究表明，补充叶酸可能在预防或减少甲基苯丙胺暴露对肾脏以及睾丸结构和功能的有害影响方面具有有益作用。未来的研究应集中于大鼠的生育结局/妊娠指数。
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