The ingestion of organophosphate (Op) pesticides for attempting suicide has been a serious health issue recently. Phorate, a class of Op, has attracted attention in this regard and so the rapid diagnosis and detection of phorate is crucial for human health. In this study, a one-step and visual method for the rapid determination of phorate via an aptasensor based on DNA-templated silver nanoclusters (DNA–AgNCs) has been presented. The designed DNA–AgNC nanoprobe, containing a special structure with an intercalated aptamer, shows excellent stability. Introducing phorate to the system easily triggers the aggregation of DNA–AgNCs, producing a brown-to-colorless color change in the solution in only 6 minutes. Under the optimal conditions, the aptasensor can allow for the determination of phorate in the concentration range of 0–25 μg mL⁻¹, using two linear equations: y = −2.308x + 0.996 (R² = 0.977) for 0–0.125 μg mL⁻¹ and y = −0.020x + 0.664 (R² = 0.971) for 0.125–25 μg mL⁻¹. The estimated detection limit of phorate was 0.012 ng mL⁻¹. Moreover, the practicability of this proposed method was also validated by analyzing phorate-spiked human blood samples and the results were in agreement with those from gas chromatography/mass spectrometry (GC/MS) analysis. Therefore, this fabricated aptasensor is suitable for sensing phorate in complex biological samples, and shows high potential for clinical diagnosis or forensic toxicology analysis.

中文翻译：

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基于DNA-AgNC aptasensor的一步，视觉和灵敏的血液中磷酸酯检测

摄入有机磷农药试图自杀已成为严重的健康问题。膦酸盐是一类Op，在这方面引起了人们的关注，因此，快速诊断和检测膦酸盐对人类健康至关重要。在这项研究中，单步和视觉方法用于快速测定甲拌磷经由提出了一种基于DNA模板的银纳米簇（DNA-AgNCs）的适体传感器。设计的DNA–AgNC纳米探针具有特殊的结构和嵌入的适体，显示出出色的稳定性。向系统中引入磷酸酯很容易触发DNA–AgNC的聚集，仅在6分钟内即可在溶液中产生从棕色到无色的颜色变化。在此条件下，在适体传感器可以允许在0-25微克毫升的浓度范围甲拌磷的确定^-1，使用两个线性方程：Ý = -2.308 X + 0.996（- [R ² = 0.977）为0-0.125微克mL ^-1和y = −0.020 x + 0.664（R ²= 0.971），适用于0.125–25μgmL ^-1。磷酸根的估计检出限为0.012 ng mL ^-1。此外，该方法的实用性还通过分析掺有血药的人体血样进行了验证，其结果与气相色谱/质谱（GC / MS）分析的结果相符。因此，这种制造的适体传感器适用于感测复杂生物样品中的磷酸根，在临床诊断或法医毒理学分析中显示出很高的潜力。