This paper describes the first immunosensor reported so far for the highly sensitive determination of the egg white allergen, ovomucoid (OM). The approach involves the selective capture of sandwich immunocomplexes formed by capture antibody-target protein allergen-HRP-labeled detector antibody onto carboxylic acid-functionalized magnetic beads (HOOC-MBs). The resulting magnetic bioconjugates were captured on the surface of disposable screen-printed carbon electrodes (SPCE) to perform amperometric detection at −0.20 V vs. the Ag pseudo-reference electrode in the presence of hydroquinone (HQ) and H₂O₂. The as prepared electrochemical immunoplatform showed a wide range of linearity (0.3–25 ng mL⁻¹), a LOD of 0.1 ng mL⁻¹, which is much lower than those claimed for commercial ELISA kits, and a great selectivity against other white egg allergenic proteins. The developed immunosensor was successfully employed for the simple and accurate determination of trace amounts of OM in unprocessed (eggs and wheat flour) and baked (bread) food samples.

本文描述了迄今为止报道的第一个用于高度灵敏地测定蛋清变应原卵粘液（OM）的免疫传感器。该方法涉及将捕获抗体-靶蛋白变应原-HRP标记的检测抗体形成的夹心免疫复合物选择性捕获到羧酸功能化磁珠（HOOC-MBs）上。将所得的磁性生物共轭物捕获在一次性丝网印刷碳电极（SPCE）的表面上，以在-0.20 V vs.的条件下进行安培检测。在存在氢醌（HQ）和H ₂ O ₂的情况下制备Ag伪参比电极。所制备的电化学免疫平台显示出广泛的线性范围（0.3–25 ng mL ^-1），LOD为0.1 ng mL ^-1，比市售ELISA试剂盒所宣称的要低得多，并且对其他白蛋过敏原蛋白的选择性也很高。研发的免疫传感器已成功用于简单，准确地测定未加工（鸡蛋和小麦粉）和烘焙（面包）食品样品中的痕量OM。