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Colorimetric detection of amoxicillin based on querecetagetin coated silver nanoparticles
Sensors and Actuators B: Chemical ( IF 8.0 ) Pub Date : 2018-03-15 , DOI: 10.1016/j.snb.2018.03.079
Noor ul Ain , Itrat Anis , Farid Ahmed , Muhammad Raza Shah , Samina Parveen , Shaheen Faizi , Shakil Ahmed

A different approach to the molecular recognition events consists in synthesis and conjugating silver nanoparticles with flavonoid quercetagetin isolated from T. erecta. In this article we are reporting rapid and feasible procedure for the synthesis of querecetagetin stabilized silver nanoparticle (Qt AgNPs). These nanoparticles were characterized by UV–vis spectroscopy, FTIR, and AFM techniques. The average size of the querecetagetin coated silver nanoparticles was found to be in the range from 25 to 45 nm and are highly robust. Querecetagetin coated silver nanoparticle selectively recognize amoxicillin producing a bathochromic shift along with color change from yellow to reddish. The detection limit was calculated using standard deviation of blank and slope of regression line and was found to be 4.46 μM with a correlation factor R2 equal to 0.993. The binding stoichiometry between Qt AgNPs and amoxicillin was found to be 1:2 (Qt AgNPs: amoxicillin) as revealed by Job‘s plot. The Qt AgNPs interact selectively with amoxicillin in human blood plasma and tap water sample showing that the ingredients of tap water and blood plasma are inert towards the interaction of Qt AgNPs with amoxicillin.Photophysical events were examined using UV–vis, FTIR spectroscopy and, AFM techniques.



中文翻译:

槲皮素涂覆的银纳米颗粒比色法检测阿莫西林

一种不同的方法,以分子识别事件在于合成,并与来自分离类黄酮quercetagetin缀合的银纳米颗粒万寿菊。在本文中,我们报告了一种快速且可行的合成槲皮素稳定银纳米颗粒(Qt AgNPs)的方法)。这些纳米颗粒通过紫外可见光谱,FTIR和AFM技术进行了表征。发现槲皮素涂覆的银纳米颗粒的平均尺寸在25至45nm的范围内,并且高度坚固。槲皮素涂层的银纳米颗粒选择性识别阿莫西林,产生红移,颜色从黄色变为红色。使用空白的标准偏差和回归线的斜率计算检出限,发现检出限为4.46μM,相关因子R 2等于0.993。如乔布的图所揭示的,发现Qt AgNP与阿莫西林之间的结合化学计量为1:2(Qt AgNP:阿莫西林)。在Qt的的AgNPs与人血浆和自来水样品中的阿莫西林选择性相互作用,表明自来水和血浆的成分对Qt AgNP与阿莫西林的相互作用呈惰性。使用紫外可见,FTIR光谱和AFM技术检查了光物理事件。

更新日期:2018-03-15
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