A sensitive and stable cellulose paper platform modified with carboxymethyl cellulose (CMC) was investigated for use as a rapid and accurate immunoassay for tuberculosis. Absorbing CMC to the paper substrate enables it to act as a linker for immobilizing biomolecules in a hydrophilic environment to promote their functionality and stability. This CMC-modified cellulose material can also be crosslinked with 1-ethyl-3-(-3-dimethylaminopropyl) carbodiimide hydrochloride/N-Hydroxy-succinimide (EDC/NHS) to generate NHS ester groups on the paper surface that can be covalently conjugated with ligands or target analytes for highly specific binding. As a result, with this system we were able to achieve a detection limit of 0.03 ng/mL for tuberculin-purified protein derivative. In addition, multiple tests can be completed simultaneously in as little as 21 min. These results demonstrate how this surface-modified paper analytical device can provide sensitive and high-throughput on-site disease diagnosis for resource-limited settings.

研究了用羧甲基纤维素（CMC）修饰的灵敏稳定的纤维素纸平台，用于结核病的快速，准确的免疫测定。将CMC吸收到纸质基材上后，它就可以充当将生物分子固定在亲水环境中的连接子，从而促进其功能性和稳定性。这种CMC改性的纤维素材料也可以与1-乙基-3-（-3-二甲基氨基丙基）碳二亚胺盐酸盐/ N交联-羟基琥珀酰亚胺（EDC / NHS）在纸张表面生成NHS酯基，可与配体或目标分析物共价缀合以实现高度特异性结合。结果，使用该系统，结核菌素纯化的蛋白衍生物的检出限为0.03 ng / mL。此外，可以在短短21分钟内同时完成多个测试。这些结果证明了这种表面改性的纸质分析设备如何为资源有限的环境提供灵敏且高通量的现场疾病诊断。