Proof of concept evidence is presented for a new method for the determination of isoaspartate, an important post-translational modification. Chemical derivatization is performed using common reagents for the modification of carboxylic acids and shown to yield suitable diagnostic information with regard to isomerization at the aspartate residue. The diagnostic gas phase chemistry is probed by collision-induced dissociation mass spectrometry, on the timescale of the MS experiment and semi-quantitative calibration of the percentage of isoaspartate in a peptide sample is demonstrated.

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中文翻译：

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气相离子化学法测定肽骨干中的异十八烷酸酯

为新的测定异天冬氨酸的方法提供了概念证据，这是一种重要的翻译后修饰。化学衍生化反应是使用普通试剂对羧酸进行修饰的，显示出可得到有关天冬氨酸残基异构化的合适诊断信息。在MS实验的时间范围内，通过碰撞诱导解离质谱法对诊断性气相化学进行了探测，并证明了肽样品中异天冬氨酸的百分比的半定量校准。

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