Antibody-antigen interactions are vital in immunoassay development and can determine detection limits and analysis times. Capillary electrophoresis (CE) is a powerful technique that can be used to quantify antibody-antigen interactions. These CE methods range from simple separations of a premixed antibody and antigen sample applied as a short plug to allow for separation of complex, free antibody, and free antigen to more complex systems which inject complexed samples in the presence of antibody or antigen; or even injections of antibody and antigen sequentially. The objective of this review is to identify and describe various CE techniques which have been used to study antibody-antigen interactions. A brief discussion of linear and nonlinear curve fitting is also included.

中文翻译：

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毛细管电泳抗体-抗原相互作用研究：综述

抗体-抗原相互作用在免疫测定开发中至关重要，可以确定检测限和分析时间。毛细管电泳 (CE) 是一种强大的技术，可用于量化抗体 - 抗原相互作用。这些 CE 方法的范围从简单分离预混合的抗体和抗原样品作为短塞，允许分离复合物、游离抗体和游离抗原，到在抗体或抗原存在的情况下注射复合样品的更复杂的系统；甚至依次注射抗体和抗原。本综述的目的是确定和描述已用于研究抗体-抗原相互作用的各种 CE 技术。还包括对线性和非线性曲线拟合的简要讨论。