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Point-of-Care Determination of Acetaminophen Levels with Multi-Hydrogen Bond Manipulated Single-Molecule Recognition (eMuHSiR)
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-03-15 00:00:00 , DOI: 10.1021/acs.analchem.7b05361 Yan Zhang 1, 2 , Zhongyuan Huang 1, 3 , Letao Wang 2 , Chunming Wang 2 , Changde Zhang 1 , Tomas Wiese 4 , Guangdi Wang 1 , Kevin Riley 1 , Zhe Wang 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-03-15 00:00:00 , DOI: 10.1021/acs.analchem.7b05361 Yan Zhang 1, 2 , Zhongyuan Huang 1, 3 , Letao Wang 2 , Chunming Wang 2 , Changde Zhang 1 , Tomas Wiese 4 , Guangdi Wang 1 , Kevin Riley 1 , Zhe Wang 1
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This work aims to face the challenge of monitoring small molecule drugs accurately and rapidly for point-of-care (POC) diagnosis in current clinical settings. Overdose of acetaminophen (AP), a commonly used over the counter (OTC) analgesic drug, has been determined to be a major cause of acute liver failure in the US and the UK. However, there is no rapid and accurate detection method available for this drug in the emergency room. The present study examined an AP sensing strategy that relies on a previously unexplored strong interaction between AP and the arginine (Arg) molecule. It was found that as many as 4 hydrogen bonds can be formed between one Arg molecule and one AP molecule. By taking advantages of this structural selectivity and high tenability of hydrogen bonds, Arg, immobilized on a graphene surface via electrostatic interactions, was utilized to structurally capture AP. Interestingly, bonded AP still remained the perfect electrochemical activities. The extent of Arg–AP bonds was quantified using a newly designed electrochemical (EC) sensor. To verify the feasibility of this novel assay, based on multihydrogen bond manipulated single-molecule recognition (eMuHSiR), both pharmaceutical and serum sample were examined. In commercial tablet measurement, no significant difference was seen between the results of eMuHSiR and other standard methods. For measuring AP concentration in the mice blood, the substances in serum, such as sugars and fats, would not bring any interference to the eMuHSiR in a wide concentration range. This eMuHSiR method opens the way for future development of small molecule detection for the POC testing.
中文翻译：
利用多氢键操纵单分子识别 (eMuHSiR) 即时测定对乙酰氨基酚水平
这项工作旨在应对当前临床环境中准确、快速监测小分子药物以进行即时 (POC) 诊断的挑战。对乙酰氨基酚 (AP) 是一种常用的非处方 (OTC) 镇痛药，过量服用已被确定是美国和英国急性肝衰竭的主要原因。然而，急诊室尚无针对该药物的快速、准确的检测方法。本研究检验了一种 AP 传感策略，该策略依赖于之前未探索过的 AP 和精氨酸 (Arg) 分子之间的强相互作用。研究发现，1个Arg分子和1个AP分子之间可以形成多达4个氢键。利用这种结构选择性和氢键的高稳定性，Arg 通过静电相互作用固定在石墨烯表面，用于结构捕获 AP。有趣的是，键合的AP仍然保持着完美的电化学活性。使用新设计的电化学 (EC) 传感器对 Arg-AP 键的程度进行量化。为了验证这种新颖测定法的可行性，基于多氢键操纵单分子识别（eMuHSiR），对药物和血清样品进行了检查。在商业片剂测量中，eMuHSiR 的结果与其他标准方法之间没有发现显着差异。在测量小鼠血液中的AP浓度时，血清中的糖、脂肪等物质在较宽的浓度范围内不会对eMuHSiR带来任何干扰。这种 eMuHSiR 方法为 POC 测试小分子检测的未来发展开辟了道路。
更新日期：2018-03-15
中文翻译：
利用多氢键操纵单分子识别 (eMuHSiR) 即时测定对乙酰氨基酚水平
这项工作旨在应对当前临床环境中准确、快速监测小分子药物以进行即时 (POC) 诊断的挑战。对乙酰氨基酚 (AP) 是一种常用的非处方 (OTC) 镇痛药，过量服用已被确定是美国和英国急性肝衰竭的主要原因。然而，急诊室尚无针对该药物的快速、准确的检测方法。本研究检验了一种 AP 传感策略，该策略依赖于之前未探索过的 AP 和精氨酸 (Arg) 分子之间的强相互作用。研究发现，1个Arg分子和1个AP分子之间可以形成多达4个氢键。利用这种结构选择性和氢键的高稳定性，Arg 通过静电相互作用固定在石墨烯表面，用于结构捕获 AP。有趣的是，键合的AP仍然保持着完美的电化学活性。使用新设计的电化学 (EC) 传感器对 Arg-AP 键的程度进行量化。为了验证这种新颖测定法的可行性，基于多氢键操纵单分子识别（eMuHSiR），对药物和血清样品进行了检查。在商业片剂测量中，eMuHSiR 的结果与其他标准方法之间没有发现显着差异。在测量小鼠血液中的AP浓度时，血清中的糖、脂肪等物质在较宽的浓度范围内不会对eMuHSiR带来任何干扰。这种 eMuHSiR 方法为 POC 测试小分子检测的未来发展开辟了道路。
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