β-glucosidase from almonds was immobilized on a polydimethylsiloxane (PDMS) microdevice by covalent chain using 3-aminopropyltrietoxysilane and glutaraldehyde. Enzymatic activity was evaluated using p-nitro-phenyl-β-D-glucopyranoside dissolved in a 0.01 M pH 5.0 phosphate solution at 45 °C measuring the reaction product (p-nitrophenol) at 410 nm. The microdevice consisted of two parts: the one part where the enzymatic reaction was carried out and a second part where pH was adjusted at 10, with NaOH. The reaction product was measured at the microchip exit using two optical fibers which were aligned facing each other with a gap of 7 mm, between both tips using guides located perpendicular to the flow outlet. A water bath was used to carry out the enzymatic reaction on the microdevice at 45 °C. The enzymatic surface of the PDMS microdevice was 1.15 cm² and the immobilized β-glucosidase amount on the microdevice was of 1.17 µg/cm². The calculated kinetics parameters were: Km 2.5 mM; Vmax 2.2 mM/min; Kcat 908.3/min and Kcat/Km 363.3/mM min. The immobilized enzyme is very stable decreasing only 5% the first 15 days; on the 30th day, the activity was 69%, regarding the initial activity.

使用3-氨基丙基三乙氧基硅烷和戊二醛通过共价链将杏仁的β-葡萄糖苷酶固定在聚二甲基硅氧烷（PDMS）微型设备上。使用对硝基苯基-β - D评估酶活性-吡喃葡萄糖苷在45°C下溶于0.01 M pH 5.0磷酸盐溶液中，在410 nm下测量反应产物（对硝基苯酚）。微型装置由两部分组成：一部分进行酶促反应，另一部分用NaOH将pH调节至10。使用两条垂直于流出口的导轨在两个尖端之间以7 mm的间隙彼此面对面对齐的两条光纤在微芯片出口处测量反应产物。使用水浴在45℃下在微型装置上进行酶促反应。PDMS微型设备的酶促表面为1.15 cm ²，固定在微型设备上的β-葡萄糖苷酶的量为1.17 µg / cm ²。计算的动力学参数为：Km2.5毫米; Vmax 2.2毫米/分钟; Kcat 908.3 / min和Kcat / Km 363.3 / mM min。固定化酶非常稳定，在开始的15天内仅下降5％；在第30天，相对于初始活动，该活动为69％。