Multiple analyte adduct formation was examined and discussed in the context of reproducible signal detection in liquid chromatography-tandem mass spectrometry applied in the analysis of biologically-related samples. Appropriate infusion solutions were prepared in H₂O/methanol (3/97, v/v) with 1 mM sodium acetate and 10 mM acetic acid. An API 4000 QTrap tandem mass spectrometer was used for experiments performed in the negative scan mode (−Q1 MS) and the negative enhanced product ion mode (-EPI). γ‑Hydroxybutyrate and its deuterated form were used as model compounds to highlight both the complexity of adduct formation in popular mobile phases used and the effective signal compensation by the application of isotope-labelled analytes as internal standards.

在用于生物相关样品分析的液相色谱-串联质谱中可再现信号检测的背景下，检查并讨论了多种分析物加合物的形成。在含有1 mM乙酸钠和10 mM乙酸的H ₂ O /甲醇（₃ /97，v / v）中制备适当的输注溶液。API 4000 QTrap串联质谱仪用于在负扫描模式（-Q1 MS）和负增强产物离子模式（-EPI）下进行的实验。γ-羟基丁酸酯及其氘代形式被用作模型化合物，以突出显示所用流行流动相中加合物形成的复杂性以及通过同位素标记的分析物作为内标物进行有效的信号补偿。