RNA is becoming more important as an increasing number of functions, both regulatory and enzymatic, are being discovered on a daily basis. As the RNA boom has just begun, most techniques are still in development and changes occur frequently. To understand RNA functions, revealing the structure of RNA is of utmost importance, which requires sample preparation. We review the latest methods to produce and purify a variation of RNA molecules for different purposes with the main focus on structural biology and biophysics. We present a guide aimed at identifying the most suitable method for your RNA and your biological question and highlighting the advantages of different methods.

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随着每天不断发现越来越多的调节功能和酶功能，RNA 变得越来越重要。由于 RNA 热潮才刚刚开始，大多数技术仍处于开发阶段，变化频繁。为了了解RNA的功能，揭示RNA的结构至关重要，这需要样品制备。我们回顾了用于不同目的生产和纯化各种 RNA 分子的最新方法，主要关注结构生物学和生物物理学。我们提供一份指南，旨在确定最适合您的 RNA 和生物学问题的方法，并强调不同方法的优点。

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