We examined the vaginal opening day, ovary and uterus organ coefficient, reproductive hormone levels of luteinizing hormone (LH) and follicle stimulating hormone (FSH), mRNA and protein expression levels of kiss-1, hypothalamus gonadotrophin releasing hormone 1 (GnRH1), organic cation transporters 2 (Oct2) and transcription termination factor 1 (Ttf1) in different pubertal ages [late lactation (18 days), pre-puberty (21–22 days), puberty (23–27 days; with respect to vaginal opening) and maturity (65 days)] of offspring females, to evaluate the effect of arsenic (As) on puberty initiation after maternal exposure to As at different concentration [0, 0.15, 1.5 and 15 mg/L As(III)] during gestational period. The results showed that the vaginal opening time was significantly advanced in utero in mice exposed to As compared to the control. The hormone level of LH was significantly increased in the mice treated with 15 mg/L of As(III) at puberty compared to the control. During puberty, the mRNA expression levels of kiss-1, GnRH1, Oct2 and Ttf1 in the hypothalamus were significantly increased in the group treated with 15 mg/L of As(III) compared to the control. The protein expressions of Kisspeptin, GnRH1, Oct2 and Ttf1 in the hypothalamus were significantly increased in the pubertal females, while Oct2 and Ttf1 expression levels were significantly decreased in the matured females compared to the control, which is in line with the transcriptional changes of related mRNA expressions. In brief, this study demonstrated that maternal exposure to As during gestational period could result in early onset of puberty in offspring females.

我们检查了阴道开放日，卵巢和子宫器官系数，黄体生成激素（LH）和卵泡刺激素（FSH）的生殖激素水平，kiss-1的mRNA和蛋白质表达水平，下丘脑促性腺激素释放激素1（GnRH1），有机阳离子转运蛋白2（Oct2）和转录终止因子1（Ttf1）在不同的青春期年龄（泌乳后期（18天），青春期前（21-22天），青春期（23-27天；关于阴道开放）和成熟期（65天）]，以评估孕妇在妊娠期砷暴露于不同浓度[0、0.15、1.5和15 mg / L As（III）]后，砷（As）对青春期开始的影响。结果表明，与对照组相比，暴露于As的小鼠子宫内的阴道开放时间明显延长。与对照组相比，在青春期用15 mg / L的As（III）处理的小鼠中LH的激素水平显着增加。在青春期期间，与对照组相比，接受15 mg / L As（III）治疗的组下丘脑中kiss-1，GnRH1，Oct2和Ttf1的mRNA表达水平显着增加。与对照组相比，青春期女性下丘脑中Kisspeptin，GnRH1，Oct2和Ttf1的蛋白表达显着增加，而成熟女性的Oct2和Ttf1的表达水平显着降低，这与相关的转录变化相符。 mRNA表达。简而言之，这项研究表明，孕期孕妇暴露于砷可能会导致后代雌性的青春期早发。