Dual Maleimide Tagging for Relative and Absolute Quantitation of Cysteine‐Containing Peptides by MALDI‐TOF MS,ChemBioChem

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Dual Maleimide Tagging for Relative and Absolute Quantitation of Cysteine‐Containing Peptides by MALDI‐TOF MS
ChemBioChem ( IF 2.6 ) Pub Date : 2018-05-02 , DOI: 10.1002/cbic.201800031
Fuzhong Ouyang ₁ , Zhihao Zhao ₁ , Ruifang Gao ₁ , Rui Shi ₁ , Enhui Wu ₁ , Rui Lv ₁ , Guoqiang Xu ₂ , Jian Liu ₁

Affiliation

Double distinction: A dual maleimide tagging method has been developed for both relative and absolute quantitation of cysteine‐containing peptides (CCPs) in combination with MALDI‐TOF mass spectrometry. The maleimides, N‐methyl‐ and N‐ethylmaleimide, with minimal differences in chemical structure, have been chosen to allow for the rapid tagging of CCPs with high efficiency.

中文翻译：

通过 MALDI-TOF MS 对含半胱氨酸肽进行双马来酰亚胺标记的相对和绝对定量

双重区别：已经开发出一种双马来酰亚胺标记方法，可结合 MALDI-TOF 质谱法对含半胱氨酸肽 (CCP) 进行相对和绝对定量。选择化学结构差异最小的马来酰亚胺、 N-甲基-和N-乙基马来酰亚胺，可以高效地快速标记 CCP。

更新日期：2018-05-02

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