An iron hydroxyl phosphate microoctahedron catalyst as an efficient peroxidase mimic for sensitive and colorimetric quantification of H2O2 and glucose,New Journal of Chemistry

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An iron hydroxyl phosphate microoctahedron catalyst as an efficient peroxidase mimic for sensitive and colorimetric quantification of H2O2 and glucose
New Journal of Chemistry ( IF 2.7 ) Pub Date : 2018-03-15 00:00:00 , DOI: 10.1039/c8nj00324f
Qimeng Yang _{1,

2,

3,

4,

5} , Shiyu Lu _{1,

2,

3,

4,

5} , Bolei Shen _{1,

2,

3,

4,

5} , Shujuan Bao _{1,

2,

3,

4,

5} , Yingshuai Liu _{1,

2,

3,

4,

5}

Affiliation

A colorimetric assay for H₂O₂ that is based on the use of iron hydroxyl phosphate (Fe₃(PO₄)₂(OH)₂) microoctahedrons, which are shown to be efficient peroxidase mimics, has been described. The microoctahedrons were prepared via a one-pot hydrothermal method. They catalyze H₂O₂ reduction and 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation to produce a blue product whose color can be observed visually and measured using spectrophotometry, best at 652 nm. The method was applied for the quantification of H₂O₂ with a limit of detection (LOD) of 0.17 μM. The scope of the method was further extended to the determination of glucose by using the enzyme glucose oxidase which catalyzes glucose oxidation to produce gluconic acid and H₂O₂ which was quantified as described above. The response is linear in the 5–100 μM glucose range, and the LOD is 1.2 μM. This work not only reports a new kind of peroxidase mimic, but also develops a simple, robust and sensitive colorimetric sensing platform for H₂O₂ and glucose detection in biomedicine and food industry.

中文翻译：

磷酸铁羟基八面体铁催化剂，可有效模拟过氧化物酶，对H ₂ O ₂和葡萄糖进行比色定量

已经描述了基于H ₂ O _2的比色测定法，该比色测定法是基于羟基磷酸铁（Fe ₃（PO ₄）₂（OH）₂）微八面体的使用，它被证明是有效的过氧化物酶模拟物。微型八面体是通过一锅水热法制备的。它们催化H ₂ O ₂还原和3,3'，5,5'-四甲基联苯胺（TMB）氧化，生成蓝色产物，其颜色可以目测观察并使用分光光度法测定，最佳波长为652 nm。该方法用于H ₂ O ₂的定量分析检测限（LOD）为0.17μM。该方法的范围进一步扩展至通过使用催化葡萄糖氧化以产生葡糖酸和H ₂ O ₂的酶葡萄糖氧化酶进行葡萄糖的测定，其如上所述进行了定量。在5-100μM葡萄糖范围内，响应是线性的，LOD为1.2μM。这项工作不仅报告了一种新型的过氧化物酶模拟物，而且还为生物医学和食品工业中的H ₂ O ₂和葡萄糖检测开发了一种简单，健壮和灵敏的比色传感平台。

更新日期：2018-03-15

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