Electrochemical Detection of Pseudomonas aeruginosa in Polymicrobial Environments,ChemistrySelect

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Electrochemical Detection of Pseudomonas aeruginosa in Polymicrobial Environments
ChemistrySelect ( IF 1.9 ) Pub Date : 2018-03-15 , DOI: 10.1002/slct.201800569
Clara Romero Santiveri ₁ , Hunter J. Sismaet ₂ , Martin Kimani ₂ , Edgar D. Goluch ₃

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A major cause of hospital‐acquired infections is Pseudomonas aeruginosa, a gram‐negative bacterial pathogen. This bacterium secretes a unique, redox‐active molecule known as pyocyanin that can serve as a useful biomarker for identification and detection of this opportunistic pathogen. This current study investigates the impact to pyocyanin production and ability to electrochemically distinguish pyocyanin when P. aeruginosa is co‐cultured with other bacterial pathogens. To accomplish this, P. aeruginosa was co‐cultured with up to four different clinically‐relevant bacteria typically found in a wound infection. P. aeruginosa’s production of pyocyanin was electrochemically monitored over a three day period and the results demonstrated that pyocyanin can be readily measured and that there were no observed enhancements or inhibitory effects to the production of pyocyanin. This information is valuable in the development of this sensing platform for clinical point‐of‐care diagnostics, where polymicrobial growth at the site of infection is common.

中文翻译：

微生物环境中铜绿假单胞菌的电化学检测

医院获得性感染的主要原因是铜绿假单胞菌（革兰氏阴性细菌病原体）。这种细菌分泌一种独特的，具有氧化还原活性的分子，称为绿脓素，可以用作识别和检测这种机会病原体的有用生物标记。这项当前的研究调查了铜绿假单胞菌与其他细菌病原体共培养时对绿脓素生成的影响以及电化学鉴别绿脓素的能力。为此，将铜绿假单胞菌与多达四种通常在伤口感染中发现的临床相关细菌共培养。铜绿假单胞菌在三天的时间里用电化学方法监测了其的绿脓素生成量，结果表明可以轻松地检测出绿脓素，并且没有观察到对绿脓素生成的增强或抑制作用。该信息对于开发用于临床即时诊断的传感平台非常有价值，在这种情况下，感染部位的微生物生长很普遍。

更新日期：2018-03-15

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